The three-dimensional structure of proteins. Chemical catalysis. The basic equations of enzyme kinetics. Measurement and magnitude of individual rate constants. The pH dependence of enzyme catalysis. Practical methods for kinetics and equilibria. Detection of intermediates in enzymatic reactions. Stereochemistry of enzymatic reactions. Active-site+directed and enzyme-activated irreversible inhibitors: " Affinity labels" and "Suicide Inhibitors". Copnformational change, allosteric regulation, motors, and work. Forces between molecules, and binding energies. Enzyme - substrate complementarity and the use of binding energy in catalysis. Spewcificity and editing mechanisms. Recombinant DNA technology. Protein engineering. Case studies of enzyme structure and mechanism. Protein stability. Kinetics of protein folding. Folding pathways and energy landscapes.