Ziquan Cao's Books

VEGF-mediated vascular functions in health and disease

By: Ziquan Cao

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VEGF-mediated vascular functions in health and disease

By: Ziquan Cao

Angiogenesis is essential for physiological processes including embryonic development, tissue regeneration, and reproduction. Under various pathological conditions the same angiogenic process contribute to the onset, development, and progression of many human diseases including cancer, diabetic complications, ocular disease, chronic inflammation and cardiovascular disease. Vascular endothelial growth factor (VEGF) is a key angiogenic factor for physiological and pathological angiogenesis. In addition to its strong angiogenic activity, VEGF also potently induces vascular permeability, often causing tissue edema in various pathological tissues. VEGF transduces its vascular signal through two tyrosine kinase receptors-VEGFR1 and VEGFR2, the latter being a functional receptor that mediates both angiogenic and vascular permeability effects. To study physiological and pathological functions of VEGF, we developed novel zebrafish disease models that permit us to study hypoxia-induced retinopathy and cancer metastasis processes. We have also administered anti-VEGF and anti-VEGFR specific antibodies to healthy mice to study the homeostatic role of VEGF in the maintenance of vascular integrity and its functions in various tissues and organs. Finally, using a zebrafish model, we evaluated if VEGF expression is regulated by circadian clock genes. In paper I, we developed protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1:EGFP zebrafish were placed in hypoxic water for 3-10 days with retinal neovascularization being analyzed using confocal microscopy. This model provides a unique opportunity to kinetically study the development of retinopathy in adult animals using non-invasive protocols and to assess the therapeutic efficacy of orally administered anti-angiogenic drugs. In paper II, we developed a zebrafish metastasis model to dissect the complex events of hypoxia-induced tumor cell invasion and metastasis in association with angiogenesis at the single-cell level. In this model, fluorescent DiI-labeled human or mouse tumor cells were implanted into the perivitelline cavity of 48-hour-old zebrafish embryos, which were subsequently placed in hypoxic water for 3 days. Tumor cell invasion, metastasis and pathological angiogenesis were analyzed using fluorescent microscopy in the living fish. The average experimental time for this model is 7 days. Our protocol offers an opportunity to study molecular mechanisms of hypoxia-induced cancer metastasis. In paper III, we show that systemic delivery of an anti-VEGF or an anti-VEGF receptor (VEGFR)-2 neutralizing antibody cause global vascular regression in mice. Among all examined tissues, the vasculature in endocrine glands, intestinal villi, and the uterus are most affected in response to VEGF or VEGFR-2 blockades. Pro-longed anti-VEGF treatment resulted in a significant decrease in the circulating levels of the predominant thyroid hormone, free thyroxine, but not the minimal isoform of triiodothyronine, suggesting that chronic anti-VEGF treatment impairs thyroid function. These findings provide structural and functional bases of anti-VEGF-specific druginduced side effects in relation to vascular changes in healthy tissues. In paper IV, we show that disruption of the circadian clock by constant exposure to light coupled with genetic manipulation of key genes in the zebrafish led to impaired developmental angiogenesis. A bmal1-specific morpholino inhibited developmental angiogenesis in zebrafish embryos without causing obvious nonvascular phenotypes. Conversely, a period2 morpholino accelerated angiogenic vessel growth, suggesting that Bmal1 and Period2 display opposing angiogenic effects. These results offer mechanistic insights into the role of the circadian clock in regulation of developmental angiogenesis, and our findings may be reasonably extended to other types of physiological or pathological angiogenesis. Overall, the results in this thesis provide further insight to angiogenic mechanistic properties in tissues and suggest possible novel therapeutic targets for the treatment of various angiogenesis-dependent diseases.

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Page Count

135

Publisher

Linköping University Electronic Press

Published Date

ISBN 10

9175190796

ISBN 13

9789175190792

Refined Safety Control of Unmanned Flight Vehicles via Fractional-Order Calculus

By: Ziquan Yu,Youmin Zhang,Bin Jiang,Chun-Yi Su

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Refined Safety Control of Unmanned Flight Vehicles via Fractional-Order Calculus

By: Ziquan Yu,Youmin Zhang,Bin Jiang,Chun-Yi Su

The monograph explores the safety of unmanned flight vehicles via the corresponding fault-tolerant control design methods. The authors analyse the safety control issues of unmanned flight vehicles, which include finite-time recovery against faults, concurrence of actuator faults and sensor faults, concurrence of actuator faults and wind effects, and faults encountered by a portion of unmanned flight vehicles in a distributed communication network. In addition, the commonly used simple but effective proportional-integral-derivative structure is also incorporated into the safety control design for unmanned flight vehicles. By using the fractional-order calculus, the developed safety control results are able to ensure flight safety and achieve the refined performance adjustments against faults and wind effects. The book will be of interest to 3rd/4th year undergraduate students, postgraduate and graduate students, researchers, academic staff, engineers of aircraft and unmanned flight vehicles.

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Page Count

249

Publisher

CRC Press

Published Date

ISBN 10

100383759X

ISBN 13

9781003837596

Fault-Tolerant Cooperative Control of Unmanned Aerial Vehicles

By: Ziquan Yu,Youmin Zhang,Bin Jiang,Chun-Yi Su

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Fault-Tolerant Cooperative Control of Unmanned Aerial Vehicles

By: Ziquan Yu,Youmin Zhang,Bin Jiang,Chun-Yi Su

This book focuses on the fault-tolerant cooperative control (FTCC) of multiple unmanned aerial vehicles (multi-UAVs). It provides systematic and comprehensive descriptions of FTCC issues in multi-UAVs concerning faults, external disturbances, strongly unknown nonlinearities, and input saturation. Further, it addresses FTCC design from longitudinal motions to attitude motions, and outer-loop position motions of multi-UAVs. The book’s detailed control schemes can be used to enhance the flight safety of multi-UAVs. As such, the book offers readers an in-depth understanding of UAV safety in cooperative/formation flight and corresponding design methods. The FTCC methods presented here can also provide guidelines for engineers to improve the safety of aerospace engineering systems. The book offers a valuable asset for scientists and researchers, aerospace engineers, control engineers, lecturers and teachers, and graduates and undergraduates in the system and control community, especially those working in the field of UAV cooperation and multi-agent systems.

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Page Count

226

Publisher

Springer Nature

Published Date

ISBN 10

9819976618

ISBN 13

9789819976614

VEGF-mediated vascular functions in health and disease

By: Ziquan Cao

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VEGF-mediated vascular functions in health and disease

By: Ziquan Cao

Average ratings

N/A

Write a review

Solve jigsaw puzzle

Age

Page Count

135

Publisher

Linköping University Electronic Press

Published Date

ISBN 10

9175190796

ISBN 13

9789175190792

Book's by Ziquan Cao

VEGF-mediated vascular functions in health and disease

VEGF-mediated vascular functions in health and disease

Refined Safety Control of Unmanned Flight Vehicles via Fractional-Order Calculus

Refined Safety Control of Unmanned Flight Vehicles via Fractional-Order Calculus

Fault-Tolerant Cooperative Control of Unmanned Aerial Vehicles

Fault-Tolerant Cooperative Control of Unmanned Aerial Vehicles

VEGF-mediated vascular functions in health and disease

VEGF-mediated vascular functions in health and disease

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