Lasso peptides form a growing family of fascinating ribosomally-synthesized and post-translationally modified peptides produced by bacteria. They contain 15 to 24 residues and share a unique interlocked topology that involves an N-terminal 7 to 9-residue macrolactam ring where the C-terminal tail is threaded and irreversibly trapped. The ring results from the condensation of the N-terminal amino group with a side-chain carboxylate of a glutamate at position 8 or 9, or an aspartate at position 7, 8 or 9. The trapping of the tail involves bulky amino acids located in the tail below and above the ring and/or disulfide bridges connecting the ring and the tail. Lasso peptides are subdivided into three subtypes depending on the absence (class II) or presence of one (class III) or two (class I) disulfide bridges. The lasso topology results in highly compact structures that give to lasso peptides an extraordinary stability towards both protease degradation and denaturing conditions. Lasso peptides are generally receptor antagonists, enzyme inhibitors and/or antibacterial or antiviral (anti-HIV) agents. The lasso scaffold and the associated biological activities shown by lasso peptides on different key targets make them promising molecules with high therapeutic potential. Their application in drug design has been exemplified by the development of an integrin antagonist based on a lasso peptide scaffold. The biosynthesis machinery of lasso peptides is therefore of high biotechnological interest, especially since such highly compact and stable structures have to date revealed inaccessible by peptide synthesis. Lasso peptides are produced from a linear precursor LasA, which undergoes a maturation process involving several steps, in particular cleavage of the leader peptide and cyclization. The post-translational modifications are ensured by a dedicated enzymatic machinery, which is composed of an ATP-dependent cysteine protease (LasB) and a lactam synthetase (LasC) that form an enzymatic complex called lasso synthetase. Microcin J25, produced by Escherichia coli AY25, is the archetype of lasso peptides and the most extensively studied. To date only around forty lasso peptides have been isolated, but genome mining approaches have revealed that they are widely distributed among Proteobacteria and Actinobacteria, particularly in Streptomyces, making available a rich resource of novel lasso peptides and enzyme machineries towards lasso topologies.
Full-Spectrum Responsive Photocatalytic Materials: From Fundamentals to Applications provides a comprehensive overview on the design, synthesis concepts, mechanisms, characterization techniques, and advances and limitations in applications of full-spectrum responsive photocatalytic materials. The book starts with the fundamentals of full-spectrum responsive materials. It then discusses the problems of most semiconductors that are not active in the whole solar spectrum and explains the benefits of utilizing full-spectrum responsive photocatalysts. Other sections describe examples of full-spectrum responsive photocatalysts classified by material types and provide the design principles and characterization protocols for these promising materials. Photocatalysis technology based on semiconductor materials holds great promise in various fields due to its potential advantages in energy-saving, cost and environmental impact. Maximizing the utilization of solar energy is always the target of pursuits in the areas of photocatalysis, and understanding and constructing appropriate full-spectrum (UV-VIS-NIR) responsive photocatalytic materials offer ways to better realize the practical utilization of photocatalysis. - Provides new insights into full-spectrum (UV-VIS-NIR) responsive photocatalysts and successful approaches for developing these materials - Assists readers working to develop more efficient catalysts and establish a solid structure–activity correlation - Suggests possibilities for the alteration of conventional photocatalysts to utilize the full spectrum of solar light
This book presents recent advances in three-dimensional (3D) imaging and display frameworks, encompassing three categories of 3D imaging and display technologies. The first category is nonphotorealistic 3D approaches based on conventional optical cameras to implement 3D stereoscopic observation of a scene. In the context of nonphotorealistic 3D imaging and reconstruction systems, the authors introduce general principles and also demonstrate camera calibration for 3D imaging, smart cameras, and full-link imaging methods using the optical modulation transfer function to improve imaging quality in conventional cameras. The second category is based on light-ray light field technology to achieve photorealistic 3D imaging and displays. In the context of light-ray light field systems, two approaches capable of light-ray light field 3D imaging by utilizing a camera array or a lens array are demonstrated. Accordingly, light-ray light field display approaches comprising head-mounted displays and integral displays are also introduced. The third category is also photorealistic 3D imaging and display technology, which is based on holography (i.e., diffraction or wavefront light field). In the corresponding holographic displays, the authors introduce 3D holographic displays from three elements: algorithms, devices, and systems, involving fast hologram generation algorithms, wide-viewing-angle display systems, and metasurface holography, etc. Including an investigative roadmap for future progress in optical imaging and 3D display systems, this book is essential reading for scientists and engineers in academia and industry who are interested in next-generation imaging and display concepts for 3D visual sensing systems.
This book presents the findings of the study, and offers analysis of both its methodological and policy-related implications. On the methodology side, it assesses and validates the valuation workshop approach; appraises the effect of distance on willingness to pay and the influence of the respondents’ ability to pay. From a policy perspective, the book examines the attitudes and preference of respondents on trade-offs between economic growth and ecological use.
This work investigates the development of English as a Foreign Language (EFL) learners’ generic competence in reading, writing and translation within the particular Chinese classroom context. It provides a new perspective for the current teaching and research in reading, writing, translation within the EFL contexts and offers an insightful framework for pedagogical applications in language learning and teaching. Its findings will be extremely valuable not only in local situations, but also more generally in a wider regional and global context as well. The book employs a series of research tools, including pre-research and post-research questionnaires, pre-test and post-test of reading/writing/translation, multi-faceted writing portfolios (including reflection reports), textual analysis and in-depth interviews. It involves 209 participants from a primary university in Wuhan, among whom 171 are undergraduates and 38 are postgraduates. And it draws on the analysis of such varied multi-sourced data both qualitatively and quantitatively. Genre-based teaching is playing a critical role in initiating EFL learners into the discourse community of the target language. Developing EFL learners’ generic competence is viewed as the ultimate goal in the process of teaching and learning. This monograph effectively demonstrates that like genre-based English for Specific Purposes (ESP) pedagogies, it is also possible to take advantage of already acquired genre knowledge for use in EFL learning contexts. It offers an impressive view of the direction in which genre-based applications are likely to take in the coming years.
This book contains an in-depth quantitative analysis of the development of the COVID-19 epidemic in China from its very beginning in December 2019 to early April 2020 when it was brought under control. It begins with adjustments of the official cumulative data on newly confirmed cases and deaths, removing any inconsistencies and smoothing the surges not attributable directly to the COVID-19 virus itself. It discusses the measures undertaken by the Chinese Government to control the epidemic. It examines the extent of the infection, the case mortality, and the costs to the Chinese economy in both Hubei, the province in which the first confirmed case was discovered, and the rest of the Mainland outside of Hubei. There is also an international comparison of the Chinese experience with those of other countries.
This book aims to explain the gap between Western theories and the Chinese administration reform experiences. The book provides insights into how the Chinese government can improve its efficiency and legitimacy through reforms and adapt Western theories with Chinese Characteristics. It also looks at the impact of modern technological innovation on reforms and why innovation is a critical key to the political development of China or other countries. The authors also explain how the Internet affects government efficiency. This timely book is an invaluable reference to better understand the changing theory of global public administration and its practice in developing countries and will interest researchers and policy makers in development studies and public administration and governance.
Beyond real GDP, innovative capacity is an important indicator of the economic strength of a nation. By studying innovative capacity and other indicators of success in innovation across the Group-of-Seven (G7) Countries, the East Asian Newly Industrialised Economies (EANIEs) and Mainland China, this book will systematically establish a positive relationship between innovation outputs and inputs of different economies. In doing so, it seeks to answer the question — are there laws of innovation? It seeks to identify the determinants of innovation at the economy-wide level, ascertain whether these determinants are similar across different economies, and find suitable metrics for comparing relative success in innovation across different economies. It concludes that innovation, rather than being a stroke of good fortune, comes from research and development activities conducted over a long period of time, and sheds light on future trends and areas for further research.
The experimental teaching of materials science and engineering (MSE) is important because the comprehensive applications and the practical knowledge of the professionals are not only an important way for undergraduate students to grasp the knowledge but also to understand the purpose of the study. In order to cultivate students' ability to solve complex engineering problems, more comprehensive experiments should be designed.Besides the essential basic experiments in the first few chapters, most of the experiments designed in this book are comprehensive, hence the title. This book breaks the boundaries in the experimental courses of MSE. The experiments in this book are modularized into five parts, including preliminary exploration of materials science and engineering, fundamentals of chemistry and crystallography, material properties, material preparation and treatment, and material applications. Besides the experiments, the appendices will describe the most relevant aspects of experimental safety, error, and data presentation in a general way. The contents and requirements of the experimental report are suggested. At the end of each chapter, a list of books, journal articles, and websites is provided for extended reading on the topics covered in the chapter.This book covers the main contents of experimental courses of MSE. The experiments cover the forefront of scientific research and the materials industry with appropriate modification. It intends to serve as a textbook for undergraduate students and aims to help teachers find a wide enough variety of experiments to construct in an experimental course.
Lasso peptides form a growing family of fascinating ribosomally-synthesized and post-translationally modified peptides produced by bacteria. They contain 15 to 24 residues and share a unique interlocked topology that involves an N-terminal 7 to 9-residue macrolactam ring where the C-terminal tail is threaded and irreversibly trapped. The ring results from the condensation of the N-terminal amino group with a side-chain carboxylate of a glutamate at position 8 or 9, or an aspartate at position 7, 8 or 9. The trapping of the tail involves bulky amino acids located in the tail below and above the ring and/or disulfide bridges connecting the ring and the tail. Lasso peptides are subdivided into three subtypes depending on the absence (class II) or presence of one (class III) or two (class I) disulfide bridges. The lasso topology results in highly compact structures that give to lasso peptides an extraordinary stability towards both protease degradation and denaturing conditions. Lasso peptides are generally receptor antagonists, enzyme inhibitors and/or antibacterial or antiviral (anti-HIV) agents. The lasso scaffold and the associated biological activities shown by lasso peptides on different key targets make them promising molecules with high therapeutic potential. Their application in drug design has been exemplified by the development of an integrin antagonist based on a lasso peptide scaffold. The biosynthesis machinery of lasso peptides is therefore of high biotechnological interest, especially since such highly compact and stable structures have to date revealed inaccessible by peptide synthesis. Lasso peptides are produced from a linear precursor LasA, which undergoes a maturation process involving several steps, in particular cleavage of the leader peptide and cyclization. The post-translational modifications are ensured by a dedicated enzymatic machinery, which is composed of an ATP-dependent cysteine protease (LasB) and a lactam synthetase (LasC) that form an enzymatic complex called lasso synthetase. Microcin J25, produced by Escherichia coli AY25, is the archetype of lasso peptides and the most extensively studied. To date only around forty lasso peptides have been isolated, but genome mining approaches have revealed that they are widely distributed among Proteobacteria and Actinobacteria, particularly in Streptomyces, making available a rich resource of novel lasso peptides and enzyme machineries towards lasso topologies.
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