The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research workers in the field of molecular biology. In choosing techniques for Volume 1 we obviously had to be selective and were unable to cover as broad a spectrum of techniques as we would have liked. However, the pro duction of Volume 2 has allowed us to develop the theme initiated in Volume 1 and also expand to include a wider range of subject areas. As with Volume 1, the majority of chapters relate to nucleic acid method ology, but we have also covered immunological methodology and protein 1. Obviously, we purification techniques that were not included in Volume see Volume 2 as simply a continuation of Volume 1. As with Volume 1, a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new termin ology, a glossary of terms has been included.
The Dutch Atlantic during an era (following the imperial moment of the seventeenth century) in which Dutch military power declined and Dutch colonies began to chart a more autonomous path. A revisionist history of the eighteenth-century Atlantic world, a counterpoint to the more widely known British and French Atlantic histories"--
Over the last two centuries, Europe has developed various forms of political representation from which democratic parliamentary systems gradually emerged. This book unravels the conditions, scale and impact under which political participation of common burghers and peasants emerged. Political participation in Europe before the Revolutions moved away from the traditional focus on ‘Three Estates’ which has often blurred the interpretation of popular participation’s role in societies. This book instead examines Europe’s key political variants such as high levels of commercialization and urbanization, combined with a balance of powers between competing categories of actors in society controlling relatively independent resources which lead to political participation forming across the continent. Instead of starting from any ideal type of political participation, this book focuses on the variation through time and space, its composition and activity, helps to explain the functions particular institutional settings fulfilled. The time frame 1100–1800 sheds light on the long-term evolutions such as institutional inertia and processes of oligarchizing. To reveal a correlation of economic and demographical growth with the claim of rising social classes to voice their interests. It also points to the opposite tendency: the formation of fiscalmilitary monarchical states. This book is essential reading for those interested in the formation of Europe’s political structures and students of premodern political history.
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research workers in the field of molecular biology. In choosing techniques for Volume 1 we obviously had to be selective and were unable to cover as broad a spectrum of techniques as we would have liked. However, the pro duction of Volume 2 has allowed us to develop the theme initiated in Volume 1 and also expand to include a wider range of subject areas. As with Volume 1, the majority of chapters relate to nucleic acid method ology, but we have also covered immunological methodology and protein 1. Obviously, we purification techniques that were not included in Volume see Volume 2 as simply a continuation of Volume 1. As with Volume 1, a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new termin ology, a glossary of terms has been included.
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