Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, genome sequencing, nucleic acids-based diagnostics and in techniques to analyze ancient and otherwise damaged DNA. Moreover, some diseases are related to DNA polymerase defects, and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. We have recently witnessed the discovery of an abundance of novel DNA polymerases in viruses, bacteria, archaea and eukaryotes with specialized properties whose physiological functions are only beginning to be understood. This book summarizes the current knowledge of these fascinating enzymes. It is intended for a wide audience from basic scientists, to diagnostic laboratories and to clinicians who seek a better understanding of these fascinating enzymes.
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, next generation sequencing, nucleic acids based diagnostics and in techniques to analyze ancient and otherwise damaged DNA (e.g. for forensic applications). Moreover, some diseases are related to DNA polymerase defects and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. This book focuses on (i) biology of DNA polymerases, (ii) medical aspects of DNA polymerases and (iii) biotechnological applications of DNA polymerases. It is intended for a wide audience from basic scientists, to diagnostic laboratories, to companies and to clinicians, who seek a better understanding and the practical use of these fascinating enzymes.
Written and edited by global leaders in the field, Pediatric Liver Transplantation: A Clinical Guide covers all aspects of treatment and management regarding this multifaceted procedure and unique patient population. This practical reference offers detailed, focused guidance in a highly templated, easy-to-consult format, covering everything from pre-transplantation preparation to surgical techniques to post-operative complications. - Provides an in-depth understanding of all aspects of pediatric liver transplantation, ideal for pediatric hepatologists, pediatric transplant surgeons, and others on the pediatric transplant team. - Covers all surgical techniques in detail, including split graft, living related, auxiliary, and domino. - Discusses pediatric liver transplantation consideration for an increasing number of additional metabolic, hematologic and renal conditions; breakthroughs in grafting and stem cell therapy; and techniques and present role of hepatocyte transplantation. - Uses a quick-reference templated format; each chapter includes an overview, pathophysiology, conventional management, controversies, and bulleted summary of key take-aways. - Includes state-of-the-art mini-reviews based on updated references and author experience throughout the text. - Features a full-color design with numerous algorithms, figures, and radiological and histopathological photos.
The Metaphysical Vision: Arthur Schopenhauer's Philosophy of Art and Life and Samuel Beckett's Own Way to Make Use of It expands upon the ideas and theories set forth in the author's Die eigentlich metaphysische Tätigkeit: Über Schopenhauers Ästhetik und ihre Anwendung durch Samuel Beckett, published (in German) in 1982 and hailed by Catharina Wulf in her book The Imperative of Narration (1997) as an «excellent study» and «the most thorough enquiry into Beckett and Schopenhauer.» In the last years of the twentieth century, new documents regarding Samuel Beckett's reading and thinking, especially important notebooks and letters, have become accessible to scholars. These documents show much more clearly than could ever be demonstrated previously that Beckett had a strong, lifelong interest in Schopenhauer's philosophy. There is no other philosopher to whom Beckett refers more often in his personal comments throughout the years of his writing up to his seventies; no other philosopher whose view of life and the world comes closer to the image of human existence we find in Samuel Beckett's literary work. The striking similarity in matters of world view and human life, and especially the evidence obtained from Beckett's previously unknown notebooks and letters, call for a close systematic study of the Beckett-Schopenhauer relationship. Due to its comprehensiveness and in-depth approach, The Metaphysical Vision is, and will be for many years to come, what its forerunner was for more than two decades: the most thorough enquiry into Beckett and Schopenhauer.
Ulrich Weisstein, an international authority in the fields of comparative literature and comparative arts, has been a pioneer paving the way for present-day intermedia studies. Among his broad intermedial interests opera has always held a central place. For the first time this volume makes available his major contributions to opera criticism in compact form, thus meeting a serious scholarly demand. The necessarily stringent selection of essays from Professor Weisstein's large output on opera, reflecting fifty years of involvement with the genre, is primarily governed by the wish to present texts that are representative of their author's work and, at the same time, are unlikely to be readily available through other channels. The fourteen essays collected are arranged in chronological order, some of them showing Ulrich Weisstein as an initiator of librettology, others tracing adaptive processes extending from textual sources to final operas, or investigating writer/composer collaborations. Further topics are satirical reflections on operatic activities in early-eighteenth-century Italy and practices of opera censorship, artist operas or definitions of romantic and epic opera. The essays are written in an accessible, essentially non-technical language and are expected to make both a profitable and a pleasurable reading for literary scholars as well as musicologists and general art lovers.
With one new volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of botany. The present volume includes reviews on plant physiology, genetics, taxonomy and geobotany.
Premodern societies believed in something sacred that obliged unconditionally. Modern societies rely on fallible science. Do they also need something absolute, a secular sacred? Steinvorth analyzes the writings of modern philosophers who claim that there is an absolute norm: the norm to be rational and authentic. In his view, their claim is true if it is reinterpreted. The norm is not moral, as it was thought to be, but metaphysical, and authenticity is not self-realization, but doing things for their own sake. In discussing the pros and cons of philosophical claims on absolutes, this book spreads out the rich pool of philosophical ideas and clarifies urgent contemporary questions about what can be demanded with universal validity. It argues this is not only the principle of justice, not to harm, but also a metaphysical principle by which to find meaning in life. Moreover, it points to some consequences this principle has in politics.
Astronomy and Astrophysics Abstracts. which appears in semi-annual volumes, is devoted to the re cording, summarizing and indexing of astronomical publications throughout the world. It is prepared under the auspices of the International Astronomical Union (according to a resolution adopted at the 14th General Assembly in 1970). Astronomy and Astrophysics Abstracts aims to present a comprehensive documentation of literature in all fields of astronomy and astrophysics. Every effort will be made to ensure that the average time interval between the date of receipt of the original literature and publication of the abstracts will not exceed eight months. This time interval is near to that achieved by monthly abstracting journals, com pared to which our system of accumulating abstracts for about six months offers the advantage of greater convenience for the user. Volume 3 contains literature published in 1970 and received before August IS, 1970; some older lite rature which was received late and which is not recorded in earlier volumes is also included.
This unusual collection of 49 essays gives an overview of the trends and accomplishments of synthetic organic chemistry in recent years. Unique in its approach, it deals with almost every aspect of modern synthesis. The first part of the book describes methods and reagents, with particular emphasis on rapidly developing organometallic and biooriented procedures. In the second part, these tools are applied to the syntheses of interesting target compounds and natural compounds with remarkable physiological properties. Mechanistic discussions and retrosynthetic analyses are included. More than 1000 up-to-date references help the reader to pursue the topics highlighted here. This book gives both the active researcher and the advanced student insight into the competitive atmosphere, creativity, and resourcefulness so characteristic of organic synthesis today.
Our world has evolved in such a way that we can no longer reduce it to just a market - it has also become an agora, where philosophers exchange world-views in order to understand one another. Europe has lost its position at the centre of the world and should stop pretending it holds the one true religion, philosophy, economy and science. Instead, we should turn our attention to fulfilling the dream of Erasmus reflected in his statement: "I wish to be a citizen of the world". First and foremost, we should learn to play fair when comparing different cultures and not rely on exlusively western criteria. This book explains how a comparative model, based on the paradigm-free axes of energy and information, accommodates the current world-views of Taoism, Buddhism and Rationalism - representing Chinese, Indian and Western heritages respectively - and shows how science and religion interrelate within such a global framework.
Astronomy and Astrophysics Abstracts, which appears in semi-annual volumes, is devoted to the re cording, summarizing and indexing of astronomical publications throughout the world. It aims to pre sent a comprehensive documentation of literature in all fields of astronomy and astrophysics. Every effort will be made to ensure that the average time interval between the date of receipt of the original literature and publication of the abstracts will not exceed eight months. This time interval is near to that achieved by monthly issued abstracting journals, compared to which our system of accumulating abstracts for about six months offers the advantage of greater convenience for the user. Volume 2 contains literature published in 1969 and received before March 15, 1970; some older lite rature which was received late and which is not recorded in Volume 1 is also included. The authors of papers who have sent us abstracts on request have effectively contributed to the suc cess of our service. We should like to express our gratitude to them. We acknowledge with thanks con tributions to this volume by Dr. J. Bou~a, who surveyed journals and publications in Czech language and supplied us with abstracts in English, by Dr. B. Onderlicka, Brno, for providing English ab stracts of Russian papers, and by the Commonwealth Scientific and Industrial Research Organization (C.S.I.R.O.), Sydney, for providing titles and abstracts of papers on radio astronomy.
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, next generation sequencing, nucleic acids based diagnostics and in techniques to analyze ancient and otherwise damaged DNA (e.g. for forensic applications). Moreover, some diseases are related to DNA polymerase defects and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. This book focuses on (i) biology of DNA polymerases, (ii) medical aspects of DNA polymerases and (iii) biotechnological applications of DNA polymerases. It is intended for a wide audience from basic scientists, to diagnostic laboratories, to companies and to clinicians, who seek a better understanding and the practical use of these fascinating enzymes.
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