The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.
Just two weeks after winning reelection to his ninth term in Congress, Steve King was stunned to learn the “Swamp” was poised to unleash a treacherous media blitzkrieg designed to kill his Congressional political career on the spot. The words, “They believe they can force you to resign” ring in his ears yet today. He knew Democrats and the media would pile on. Unfortunately, the threat was from within his party and it was far more dangerous. The Republican establishment, RINOs, elitists, globalists, and NeverTrumpers needed him out of the way. This is the full story.
Steve Shone’s Women of Liberty explores the many overlaps between ten radical, feminist, and anarchist thinkers: Tennie C. Claflin, Noe Itō, Louise Michel, Rose Pesotta, Margaret Sanger, Elizabeth Cady Stanton, Mollie Steimer, Lois Waisbrooker, Mercy Otis Warren, and Victoria C. Woodhull. In an age of great and understandable dissatisfaction with governments around the world, Shone illuminates both the lost wisdom of the anarchists and the considerable contribution of women to intellectual thought, influences that are currently missing from many classes documenting the history of political theory.
“A valuable and illuminating read, shedding a lot of light on the political, economic and technological factors that have driven circus evolution” (The Circus Diaries). Beneath the Big Top is a social history of the circus, from its ancient roots to the rise of the “modern” tented travelling shows. A performer and founder of a circus group, Steve Ward draws on eyewitness accounts and contemporary interviews to explore the triumphs and disasters of the circus world. He reveals the stories beneath the big top during the golden age of the circus and the lives of circus folk, which were equally colorful outside the ring: • Pablo Fanque, Britain’s first black circus proprietor • The Chipperfield dynasty, who started out in 1684 on the frozen Thames • Katie Sandwina, world’s strongest woman and part-time crime-fighter • The Sylvain brothers, who fell in love with the same woman in the ring “As a former circus performer and now teacher and circus professional I thoroughly enjoyed this book!! The Circus has such a rich history and Steve does an amazing job at not only chronicling it but also telling entertaining and wonderful stories throughout. The photos are also amazing!! I recommend this book for both circus professionals and also for everyone else . . . it is a fabulous read for all!!” —Carrie Heller, Circus Arts Institute (Atlanta, GA)
Steve Haydock joined the British Army in 1972 aged 15, joining the lnfantry Junior Leaders Battalion, based in Oswestry, Shropshire. On his 18th birthday he arrived in Northern lreland to begin the first of his three tours of duty in the province, with the 1st Battalion of The Queens Lancashire Regiment. He served nine years with the QLR from Northern Ireland to Cyprus to Ghana before becoming a civilian in the mid 1980's. In 1992, after seeing the war unfold in Yugoslavia he left England to join the Croatian Army, to use his experience and skills to help the Croats fight to defend the country and win freedom from the Serbian aggressor. This is his story................
This book explores the ideas of three largely forgotten radical women who participated in labor union strikes in Argentina and Uruguay, Canada, and the United States: Virginia Bolten (c.1876-1960), one of the most militant anarchists of southern South America; Helen Armstrong (1875-1947), a major leader of the Winnipeg General Strike of 1919, whose involvement in that important event in Canadian history was, for a long time, obscured by accounts that emphasized the accomplishments of men; and Elizabeth Gurley Flynn (1890-1964), the Wobbly leader who directed many industrial strikes throughout the United States, and was one of the founders of the American Civil Liberties Union, who eventually became the leader of the Communist Party, USA. It also examines the contributions of two similarly neglected anarchist men who participated in labor union strikes and industrial action in New Zealand, Australia, Chile, Argentina, and Japan. Tom Barker (1887-1970) was an anarchist who eventually became a socialist who worked to promote labor unionism on four continents and who tried to create a global One Big Union for sailors. Kōtoku, Shūsui (1871-1911) was a liberal who became a socialist and finally an anarchist. An opponent of governmental imperialism and ecological mismanagement, he studied and translated the works of Western thinkers and sought to apply what he learned from other cultures to the development of Japan.
In this full-length study of the 1913 Paterson silk strike, Steve Golin examines the creative collaboration between the silk workers, organizers from the Industrial Workers of the World, and Greenwich Village intellectuals. Although the strike was defeated, this alliance could become a model for the American left because it suggests the possibilities of connecting economic, political, and cultural struggles.Combining perspectives from labor history, social history, and intellectual history Golin argues that while the silk workers began the 1913 strike and controlled it themselves, the IWW helped them create institutions that supported the strike and reinforced its radically democratic character. The deadlock in Paterson dictated the need for a "bridge" to New York that was facilitated by a growing mutual trust between the Wobblies and intellectuals from Greenwich Village. At the height of the struggle, the IWW and the Village radicals joined the workers in presenting a powerful strike pageant in Madison Square Garden.The story of the 1913 silk strike is important because it challenges long-held conservative assumptions about labor history, including the elitist role of skilled workers, the bureaucratic function of union organization, and the irrelevance of intellectuals. Although the strikers were ultimately defeated, the strike's failure had more damaging consequences for the IWW and the intellectuals than for the workers themselves and Golin views this loss as a major turning point for the American left. Author note: Steve Golin is Professor of History at Bloomfield College in New Jersey.
A thrilling narrative of scientific triumph, decades of secrecy, and the unimaginable destruction wrought by the creation of the atomic bomb. It began with plutonium, the first element ever manufactured in quantity by humans. Fearing that the Germans would be the first to weaponize the atom, the United States marshaled brilliant minds and seemingly inexhaustible bodies to find a way to create a nuclear chain reaction of inconceivable explosive power. In a matter of months, the Hanford nuclear facility was built to produce and weaponize the enigmatic and deadly new material that would fuel atomic bombs. In the desert of eastern Washington State, far from prying eyes, scientists Glenn Seaborg, Enrico Fermi, and many thousands of others—the physicists, engineers, laborers, and support staff at the facility—manufactured plutonium for the bomb dropped on Nagasaki, and for the bombs in the current American nuclear arsenal, enabling the construction of weapons with the potential to end human civilization. With his characteristic blend of scientific clarity and storytelling, Steve Olson asks why Hanford has been largely overlooked in histories of the Manhattan Project and the Cold War. Olson, who grew up just twenty miles from Hanford’s B Reactor, recounts how a small Washington town played host to some of the most influential scientists and engineers in American history as they sought to create the substance at the core of the most destructive weapons ever created. The Apocalypse Factory offers a new generation this dramatic story of human achievement and, ultimately, of lethal hubris.
Exam Board: AQA Level: AS/A-level Subject: History First Teaching: September 2015 First Exam: June 2016 AQA approved Enhance and expand your students' knowledge and understanding of their AQA breadth study through expert narrative, progressive skills development and bespoke essays from leading historians on key debates. - Builds students' understanding of the events and issues of the period with authoritative, well-researched narrative that covers the specification content - Introduces the key concepts of change, continuity, cause and consequence, encouraging students to make comparisons across time as they advance through the course - Improves students' skills in tackling interpretation questions and essay writing by providing clear guidance and practice activities - Boosts students' interpretative skills and interest in history through extended reading opportunities consisting of specially commissioned essays from practising historians on relevant debates - Cements understanding of the broad issues underpinning the period with overviews of the key questions, end-of-chapter summaries and diagrams that double up as handy revision aids The Making of a Superpower: USA 1865-1975 This title explores the era of reconstruction, the development of the US as an economic power, its role in international affairs, domestic issues and its emergence as a superpower. It considers breadth issues of change, continuity, cause and consequence in this period through examining key questions on themes such as governance, economy, society, the role of the USA in world affairs, ideology and the impact of key individuals.
Why does the Republican Party always seem to betray conservatives in the end? How come Leftists never worry some “squishy” moderate will win Democrat primaries, even though conservatives in the GOP are constantly fighting that battle? Why do GOP leaders typically fight the conservative base harder than they fight the Democrats? How did the Left gain control of every major cultural institution in the country? Why does the Republican Party continue to fund (alleged) political enemies, such as Planned Parenthood? Is the GOP really the “the stupid party” or something much worse? Deace answers these questions and more in Truth Bombs, a definitive expose on why conservativism has no future—if it remains hostage to the unibrow-party duopoly.
One of the oldest cities in America, Hartford holds plenty of sinful stories. Famed inventor and industrialist Samuel Colt sold arms to both the North and South in the buildup to the Civil War. The notorious Seyms Street jail was the subject of national criticism and scandal for its deplorable conditions. Local journalist Daniel Birdsall fought to expose corruption in the powerful insurance industry and local government at the expense of his own printing presses. Tension between unions and "robber barons" such as Jay Gould spilled into the streets during the Gilded Age. Author Steve Thornton takes readers on an exciting journey through the seedy underbelly of Hartford's past.
Scholars have long described modernism as "heretical" or "iconoclastic" in its assaults on secular traditions of form, genre, and decorum. Yet critics have paid surprisingly little attention to the related category of blasphemy--the rhetoric of religious offense--and to the specific ways this rhetoric operates in, and as, literary modernism. United by a shared commitment to "the word made flesh," writers such as James Joyce, Mina Loy, Richard Bruce Nugent, and Djuna Barnes made blasphemy a key component of their modernist practice, profaning the very scriptures and sacraments that fueled their art. In doing so they belied T. S. Eliot's verdict that the forces of secularization had rendered blasphemy obsolete in an increasingly godless century ("a world in which blasphemy is impossible"); their poems and fictions reveal how forcefully religion endured as a cultural force after the Death of God. More, their transgressions spotlight a politics of religion that has seldom engaged the attention of modernist studies. Blasphemy respects no division of church and state, and neither do the writers who wield it to profane all manner of coercive dogmas--including ecclesiastical as well as more worldly ideologies of race, class, nation, empire, gender, and sexuality. The late-century example of Salman Rushdie's The Satanic Verses affords, finally, a demonstration of how modernism persists in postwar anglophone literature and of the critical role blasphemy plays in that persistence. Blasphemous Modernism thus resonates with the broader cultural and ideological concerns that in recent years have enriched the scope of modernist scholarship.
In the spring of 2001, George W. Bush selected Dallas attorney Robert W. Jordan as the ambassador to Saudi Arabia. Jordan's nomination sped through Congress in the wake of the terrorist attacks on 9/11, and he was at his post by early October, though with no prior diplomatic experience, as Saudi Arabia mandates that the U.S. Ambassador be a political appointee with the ear of the president. Hence Jordan had to learn on the job how to run an embassy, deal with a foreign culture, and protect U.S. interests, all following the most significant terrorist attacks on the United States in history.
Recent advances in next-generation sequencing have enabled high-throughput determination of biological sequences in microbial communities, also known as microbiomes. The large volume of data now presents the challenge of how to extract knowledge—recognize patterns, find similarities, and find relationships—from complex mixtures of nucleic acid sequences currently being examined. In this chapter we review basic concepts as well as state-of-the-art techniques to analyze hundreds of samples which each contain millions of DNA and RNA sequences. We describe the general character of sequence data and describe some of the processing steps that prepare raw sequence data for inference. We then describe the process of extracting features from the data, assigning taxonomic and gene labels to the sequences. Then we review methods for cross-sample comparisons: (1) using similarity measures and ordination techniques to visualize and measure differences between samples and (2) feature selection and classification to select the most relevant features for discriminating between samples. Finally, in conclusion, we outline some open research problems and challenges left for future research.
Find FREE chapter quizzes online Discover important events that shaped the nation Get to know the superstars of the past Don't miss a moment of U.S. history The United States is undergoing a period of intense political and social change. From the rise of the Tea Party to social media's effect on American life and politics, this new edition fills in the gaps of this nation's story. This book guides you through the events that shaped the nation, from pre-Columbian civilizations to the 21st century. It's all here—you'll find all the wars, leaders, and eras that explain and demonstrate how the past influences the future. Inside... Get an overview of U.S. history Learn about major movements Discover how the U.S. came of age Explore iconic cultural moments Find out how the country faced adversity Get to know historical U.S. documents FREE 1-year access to chapter quizzes online!
America's broken food system has provoked an outcry from consumer advocates seeking to align food policies with public health objectives. This book examines both sides of the conflict for solutions. Many believe that America's food system is in dire need of reform, with concerns ranging from the obesity epidemic to exploitative labor practices and negative environmental impact. This eye-opening book answers provocative questions about what changes are needed, who is advocating the changes, what parties are opposing these changes (and why), and what a new food system would look like. Organized into three sections, the work identifies the problems with the current system, reviews the changing landscape of food policy, and suggests workable solutions for progress. Washington insider Steve Clapp takes a comprehensive look at the struggle over the future of food. He examines the vision for a reformed national food policy that includes calculating the true cost of food, providing universal access to healthful food, adopting farm policies supporting public health and environmental objectives, improving food safety, paying fair wages to food employees, treating food animals with compassion, and reducing the food system's carbon footprint. The book explores the ways in which these issues can be resolved, drawing upon lessons learned from the early food advocates of the 1960s and 1970s.
Critics have raved about this delightful book Allen addresses the muddleheadedness (or "dumbth") that pervades all segments of American life and work, and offers 81 "rules" for good thinking.
This book explains the five pillars or battlefields of cybersecurity and how a Zero Trust approach can change the advantage on each battlefield. We have taken a deep dive into each of five battlefields where we have a decided disadvantage due to constitutional structure and moral behavioral guidelines, where we provide examples of how we got here, what we can do about it, why we got here, and how we can avoid these traps in the future. This is a unique viewpoint that has never been explored – the five battlefields include Economics, Technology, Information, Education, and Leadership – and how each has contributed to our current disadvantage on the global stage. We go on to discuss how Zero Trust can change the game to create an advantage for us going forward. The credibility of Zero Trust stems directly from the father of Zero Trust, John Kindervag, who says, “And now, Steve has written a new book on Zero Trust called Losing the Cybersecurity War: And What We Can Do to Stop It. It is undeniably the best Zero Trust book yet written. While other writers have focused on implementing Zero Trust from their perspectives, Steve focuses on why Zero Trust is so important on the modern cybersecurity battlefield. His concept of the five cyber battlefields is a great insight that will help us win the cyberwar. By weaving Zero Trust principles throughout these five concepts, Steve demonstrates how the ideas and efforts involved in building Zero Trust environments will lead to a profound shift in terrain advantage. No longer will attackers own the high ground. As defenders and protectors, we can leverage modern technology in a Zero Trust way to keep our data and assets safe from infiltration and exploitation.”
One of the most interesting topics in the Bible is the doctrine of the Second Coming of Christ. God has not left us in the dark concerning the details of Christ's future coming, but has given us several clear prophetic signs to watch for. Signs of the Second Coming of Jesus Christ and the End of the World looks at key events the Bible says will lead to the glorious return of the Lord Jesus at the end of the age. If you ever wondered why the world is the way it is, and where it is headed, then you need to read this book! Whether youare a Christian or not, this revealing work will give you the vital information you need to prepare for what may come in the near future, and for eternity itself. (this book gives much historical information, comparesworldviews, and provides several charts for a better understanding of historical developments).
Understand the clinically relevant aspects of microbiology with this student-acclaimed, full-color review --- bolstered by case studies and hundreds of USMLE®-style review questions A Doody's Core Title for 2024 & 2021! Since 1954, Jawetz, Melnick & Adelberg’s Medical Microbiology has been hailed by students, instructors, and clinicians as the single-best resource for understanding the roles microorganisms play in human health and illness. Concise and fully up to date, this trusted classic links fundamental principles with the diagnosis and treatment of microbial infections. Along with brief descriptions of each organism, you will find vital perspectives on pathogenesis, diagnostic laboratory tests, clinical findings, treatment, and epidemiology. The book also includes an entire chapter of case studies that focuses on differential diagnosis and management of microbial infections. Here’s why Jawetz, Melnick & Adelberg’s Medical Microbiology is essential for USMLE® review: 640+ USMLE-style review questions 350+ illustrations 140+ tables 22 case studies to sharpen your differential diagnosis and management skills An easy-to-access list of medically important microorganisms Coverage that reflects the latest techniques in laboratory and diagnostic technologies Full-color images and micrographs Chapter-ending summaries Chapter concept checks Jawetz, Melnick & Adelberg’s Medical Microbiology, Twenty-Eighth Edition effectively introduces you to basic clinical microbiology through the fields of bacteriology, mycology, and parasitology, giving you a thorough yet understandable review of the discipline. Begin your review with it and see why there is nothing as time tested or effective.
Described by Richard Sherwin of New York Law School as the law and film movement's 'founding text', this text is a second, heavily revised and improved edition of the original Film and the Law (Cavendish Publishing, 2001). The book is distinctive in a number of ways: it is unique as a sustained book-length exposition on law and film by law scholars; it is distinctive within law and film scholarship in its attempt to plot the parameters of a distinctive genre of law films; its examination of law in film as place and space offers a new way out of the law film genre problem, and also offers an examination of representations of an aspect of legal practice, and legal institutions, that have not been addressed by other scholars. It is original in its contribution to work within the wider parameters of law and popular culture and offers a sustained challenge to traditional legal scholarship, amply demonstrating the practical and the pedagogic, as well as the moral and political significance of popular cultural representations of law. The book is a valuable teaching and learning resource, and is the first in the field to serve as a basic guidebook for students of law and film.
Gain a practical prescription for both private and public organizations to remediate threats and maintain a competitive pace to lead and thrive in an ever-shifting environment. In today’s hyper-connected, always-on era of pervasive mobility, cloud computing and intelligent connected devices, virtually every step we take, every transaction we initiate, and every interaction we have are supported in some way by this vast global infrastructure. This set of interconnected systems comprises the fundamental building blocks of the second economy – the very foundation of our first economy. And adversaries, whether motivated by profit, principle or province, are singularly focused on winning the race through a relentless portfolio of shifting attack vectors. Make no mistake about it, we are running a race. This is a race against a faceless, nameless adversary – one that dictates the starting line, the rules of the road, and what trophies are at stake. Established assumptions must be challenged, strategies must be revised, and long-held practices must be upended to run this race and effectively compete. The Second Economy highlights a second to none approach in this fight, as the effectiveness and ROI of security solutions are increasingly measured by the business outcomes they enable. What You Will Learn: Understand the value of time and trust in a cyber-warfare world Enable agile and intelligent organizations to minimize their risk of falling victim to the next attack Accelerate response time by adopting a holistic approach Eliminate friction across the threat defense lifecycle, from protection to detection to correction Gain a sustainable competitive advantage by seizing first mover advantage Deploy solutions across an open, integrated security framework Who This Book Is For: Senior-level IT decision makers concerned with ascribing business value to a robust security strategy. The book also addresses business decision makers who must be educated about the pervasive and growing cyber threatscape (including CXOs, board directors, and functional leaders) as well as general business employees to understand how they may become unwitting participants in a complex cyber war.
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