Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. - Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments - Up-to-date description of normalization methods and current methods for sample amplification and labeling - Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis - Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays
This comprehensive overview of local food systems explores alternative definitions of local food, estimates market size and reach, describes the characteristics of local consumers and producers, and examines early indications of the economic and health impacts of local food systems. Defining ¿local¿ based on marketing arrangements, such as farmers selling directly to consumers at regional farmers¿ markets or to schools, is well recognized. Statistics suggest that local food markets account for a small, but growing, share of U.S. agricultural production. For smaller farms, direct marketing to consumers accounts for a higher percentage of their sales than for larger farms. Charts and tables.
Founded by Walter Williams, a newsman who lacked a college education, the University of Missouri's School of Journalism is regarded as among the best in the world. Weinberg uncovers the history of the school's first 100 years, revealing the flaws as well as the virtues of the Missouri Method"--Provided by publisher.
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