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Techniques for Biochemical Analysis

By: Khursheed Hussain,Sameena Maqbool Lone,Khalid Z. Masoodi,Syed Minu Balkhi

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Techniques for Biochemical Analysis

By: Khursheed Hussain,Sameena Maqbool Lone,Khalid Z. Masoodi,Syed Minu Balkhi

Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more. The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects. - Features step-by-step methods for biochemical analysis of a variety of compounds - Explores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areas - Provides detailed instructions on how to prepare buffers, the equipment to be used, and the analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormones - Contains interdisciplinary and adaptable methods and techniques

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Page Count

258

Publisher

Elsevier

Published Date

ISBN 10

0443159157

ISBN 13

9780443159152

Advanced Methods in Molecular Biology and Biotechnology

A Practical Lab Manual

By: Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool

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Advanced Methods in Molecular Biology and Biotechnology

A Practical Lab Manual

By: Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

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Page Count

220

Publisher

Academic Press

Published Date

ISBN 10

012824450X

ISBN 13

9780128244500