Oxophytodienoic acid reductases (OPRs) are flavoenzymes closely related to Old Yellow Enzyme (OYE) from Saccharomyces. The physiological role of plant OPRs could only be clarified for OPR3: OPR3 from tomato and Arabidopsis reduce the double bond of the α,β-unsaturated carbonyl group of (9S,13S)-oxophytodienoic acid (OPDA), the precursor of the phytohormone jasmonic acid (JA). OPR3 is therefore an important step for JA biosynthesis and the following JA-triggered defensive and developmental adaptations of the plant. Since the production of phytohormones, including JA, is regulated in an extremely time- and tissue specific manner, the regulatory step of JA-biosynthesis was sought. The conversion of OPDA by OPR3 was proposed as the rate-limiting step in biosynthesis as OPR3 turned out to form a self-inhibiting dimer when crystallized. In the OPR3 crystal, the L6-loop from each protomer reaches into the active site cavity of the other protomer. The dimerization-dependent block of the active site provides a hypothetical mechanism for the regulation of OPR3 activity. Interestingly, two sulfate ions were enclosed in the interacting site of the protomers, suggesting that the dimer might be stabilized in vivo by reversible sulfation or phosphorylation of the tyrosine 364(SlOPR3) or 365 (AtOPR3), respectively. The role of this hypothesized sulfation/phosphorylation was subject of this study. Neither sulfation nor phosphorylation of Y365 could be detected by mass spectrometry. Hence, studies were continued with an in vitro approach where OPR3 was expressed with sulfotyrosine incorporated co-translationally at position 365 (Y365SY). Biochemical characterization led to contradictory results: On the one hand, interaction strength of Y365SY was unaltered in comparison to wild-type OPR3, while on the other hand, activity of Y365SY was reduced. Closer examination indicated that substrate binding or product release was reduced in Y365SY. These changes could be traced back to the additional charge of the SO42—ion, which leads to a narrowing of the entrance to the active site cavity. With this finding, the proposed regulating mechanism by sulfation/phosphorylation is still valid, but independent from dimerization. In order to link this potential regulatory mechanism with a post-translational modification in vivo, an untargeted screen was performed, in which OPR3 was expressed as a fusion protein with a promiscous biotin ligase (BioID2). With this method, potentially interacting proteins were biotinylated in vivo and subsequently isolated and analyzed by MS/MS. Many candidate proteins were identified for OPR3, including kinases and phosphatases. Additionally, OPR1, OPR2 and OPR4 from Arabidopsis were also expressed as BioID2 fusion proteins in order to clarify their physiological role. The most promising results were obtained for OPR4, which was found to be association with stress granule and P-body proteins.
First published in 1988, this encyclopedia serves as an overview and point of entry to the complex interdisciplinary field of Victorian studies. The signed articles, which cover persons, events, institutions, topics, groups and artefacts in Great Britain between 1837 and 1901, have been written by authorities in the field and contain bibliographies to provide guidelines for further research. The work is intended for undergraduates and the general reader, and also as a starting point for graduates who wish to explore new fields.
The rise of performance art as chronicled by renowned critic Sally Banes. Her approach to the complex matrix of art, community, and culture draws on histories and theories of painting, photography, dance, theater, and folklore. Her vivid descriptions and provocative interpretations fill a gap in the history of contemporary performance--where the avant-garde met the mainstream.
Since the early 1980s, Australian governments have pursued policies of 'international competitiveness' that combine the removal of tariffs and other industry protection with incentives to restructure the economy and encourage industries in which Australia enjoys a comparative advantage." -- Cover.
Top experts from Vanderbilt University School of Nursing have put together an excellent issue devoted to Geriatric Syndromes that will prepare the reader for treatment and patient care of geriatric patients. Top authors have written reviews in the following areas: Cognitive Issues; GI Disturbances; Urinary Incontinence; Frailty; Impaired Mobility and Functional Decline; Risk for Injury (Falls); Nutritional Risks; Pain Management; Polypharmacy Management; Impairments in Skin Integrity; and Sleep Disorders. Nurses will come away with a current view of the clinical management for these clinical issues in geriatric population.
Now in its fourth edition trusted textbook Older People: Issues and Innovations in Care provides a unique collection of conversations and commentaries by leading international and local experts on a range of contemporary issues around the care of older people. Featuring six new chapters, current research and policy changes, the esteemed author team continue to highlight the importance of interdisciplinary healthcare in providing a comprehensive, person-centred approach to care. This edition encourages readers to explore care issues, innovations and change, and to utilise evidence-based practice to improve the care of older people and their families. - Editors' comments precede each chapter, providing a snapshot of the issues addressed. - Dementia care has an increased focus. New chapters include: - Caring for older people: issues for consumers - Younger people in residential aged care facilities - Health and care of older Aboriginal and Torres Strait Islander peoples - Alzheimer's dementia: neuropsychology, early diagnosis and intervention - Self-esteem, dignity and finding meaning in dementia - My journey of heartbreak: my parents and Alzheimer's disease. - Vignettes highlight innovative approaches to care that result in improved health outcomes for older people. - Key points are woven through the text to reiterate vital information relevant to nurses and aged care workers. - Reflective questions encourage critical thinking as an instrument for improving practice. - In-text references are made to video interviews available on the Evolve site. This text reflects new thinking in care; include the ideas and experiences of policy analysts, nurses, doctors, allied health professionals and the consumer experience mainly from Australia but with international contributions and be based on contemporary research. It will also point readers to 'the evidence' where it exists, and include vignettes of practice and 'video' clips where appropriate.
This book examines the process of ego development in adolescence. It explores the diverse ways in which mothers and fathers subtly direct their teenagers on to one of the four main paths through adolescence, and facilitate or impede their development - and the equally diverse ways in which teenager's interactions with their parents may affect the parents. Throughout, choices of real children and parents are presented - some happy and successful, others troubled. The book is aimed at those who work professionally with adolescents and their families.
In a quiet town of Seneca Falls, New York, over the course of two days in July, 1848, a small group of women and men, led by Elizabeth Cady Stanton and Lucretia Mott, held a convention that would launch the woman's rights movement and change the course of history. The implications of that remarkable convention would be felt around the world and indeed are still being felt today. In Seneca Falls and the Origins of the Woman's Rights Movement, the latest contribution to Oxford's acclaimed Pivotal Moments in American History series, Sally McMillen unpacks, for the first time, the full significance of that revolutionary convention and the enormous changes it produced. The book covers 50 years of women's activism, from 1840-1890, focusing on four extraordinary figures--Lucretia Mott, Elizabeth Cady Stanton, Lucy Stone, and Susan B. Anthony. McMillen tells the stories of their lives, how they came to take up the cause of women's rights, the astonishing advances they made during their lifetimes, and the lasting and transformative effects of the work they did. At the convention they asserted full equality with men, argued for greater legal rights, greater professional and education opportunities, and the right to vote--ideas considered wildly radical at the time. Indeed, looking back at the convention two years later, Anthony called it "the grandest and greatest reform of all time--and destined to be thus regarded by the future historian." In this lively and warmly written study, Sally McMillen may well be the future historian Anthony was hoping to find. A vibrant portrait of a major turning point in American women's history, and in human history, this book is essential reading for anyone wishing to fully understand the origins of the woman's rights movement.
Now in its fourth edition trusted textbook Older People: Issues and Innovations in Care provides a unique collection of conversations and commentaries by leading international and local experts on a range of contemporary issues around the care of older people. Featuring six new chapters, current research and policy changes, the esteemed author team continue to highlight the importance of interdisciplinary healthcare in providing a comprehensive, person-centred approach to care. This edition encourages readers to explore care issues, innovations and change, and to utilise evidence-based practice to improve the care of older people and their families. - - Editors' comments precede each chapter, providing a snapshot of the issues addressed. - - Dementia care has an increased focus. - New chapters include: - - Caring for older people: issues for consumers - - Younger people in residential aged care facilities - - Health and care of older Aboriginal and Torres Strait Islander peoples - - Alzheimer's dementia: neuropsychology, early diagnosis and intervention - - Self-esteem, dignity and finding meaning in dementia - - My journey of heartbreak: my parents and Alzheimer's disease. - - Vignettes highlight innovative approaches to care that result in improved health outcomes for older people. - - Key points are woven through the text to reiterate vital information relevant to nurses and aged care workers. - - Reflective questions encourage critical thinking as an instrument for improving practice. - - In-text references are made to video interviews available on the Evolve site.
As an international ecotourism destination, Yosemite National Park welcomes millions of climbers, sightseers, and other visitors from around the world annually, all of whom are afforded dramatic experiences of the natural world. This original and cross-disciplinary book offers an ethnographic and performative study of Yosemite visitors in order to understand human connection with and within natural landscapes. By grounding a novel “eco-semiotic” analysis in the lived reality of parkgoers, it forges surprising connections, assembling a collective account that will be of interest to disciplines ranging from performance studies to cultural geography.
An accessible narrative biography, Frances Power Cobbe traces the details of Cobbe's life and work, analyzes her writing, and sets both in the context of the social and intellectual debates of her time.
- Aligned to the 2020 ACORN Standards - Engaging patient scenarios woven through the text, include patient histories and indications for surgery - Information on managing surgery during pandemics, including COVID 19 - Details of the extended roles available in perioperative practice
This will help us customize your experience to showcase the most relevant content to your age group
Please select from below
Login
Not registered?
Sign up
Already registered?
Success – Your message will goes here
We'd love to hear from you!
Thank you for visiting our website. Would you like to provide feedback on how we could improve your experience?
This site does not use any third party cookies with one exception — it uses cookies from Google to deliver its services and to analyze traffic.Learn More.