Major parts of the oceans and lands of our planet are permanently, or temporarily, exposed to temperatures below 10 C. Microorganisms, plants and animals living under these conditions have adapted to their environments in such a way that metabolic processes, reproduction and survival strategies are optimal for their natural biotopes. This book presents the most recent knowledge of the ecology and the physiology of cold-adapted microorganisms, plants and animals, and explains the mechanisms of cold-adaptation on the enzymatic and molecular level, including results from the first crystal structures of enzymes of cold-adapted organisms.
Most of the Earth’s biosphere is characterized by low temperatures. Vast areas (>20%) of the soil ecosystem are permanently frozen or are unfrozen for only a few weeks in summer. Permafrost regions occur at high latitudes and also at high ele- tions; a significant part of the global permafrost area is represented by mountains. Permafrost soils are of global interest, since a significant increase in temperature is predicted for polar regions. Global warming will have a great impact on these soils, especially in northern regions, since they contain large amounts of organic carbon and act as carbon sinks, and a temperature increase will result in a release of carbon into the atmosphere. Additionally, the intensified release of the clima- relevant tracer gas methane represents a potential environmental harzard. Significant numbers of viable microorganisms, including bacteria, archaea, p- totrophic cyanobacteria and green algae, fungi and protozoa, are present in per- frost, and the characteristics of these microorganisms reflect the unique and extreme conditions of the permafrost environment. Remarkably, these microorg- isms have been reported to be metabolically active at subzero temperatures, even down to ?20°C.
This volume presents detailed descriptions of methods for evaluating, monitoring and assessing bioremediation of soil contaminated with organic pollutants or heavy metals. Traditional soil investigation techniques, including chemical, physical and microbiological methods, are complemented by the most suitable modern methods, including bioreporter technology, immunological, ecotoxicological and molecular assays. Step-by-step procedures, lists of required equipment and reagents and notes on evaluation and quality control allow immediate application
Major parts of the oceans and lands of our planet are permanently, or temporarily, exposed to temperatures below 10 C. Microorganisms, plants and animals living under these conditions have adapted to their environments in such a way that metabolic processes, reproduction and survival strategies are optimal for their natural biotopes. This book presents the most recent knowledge of the ecology and the physiology of cold-adapted microorganisms, plants and animals, and explains the mechanisms of cold-adaptation on the enzymatic and molecular level, including results from the first crystal structures of enzymes of cold-adapted organisms.
There is an increasing interest of biotechnologists in the potential of cold-adapted organisms, since they play a major role in the processes of nutrient turnover and primary biomass production in cold ecosystems. Essential advantages of the application of such organisms are the rapid microbial metabolism at low temperatures, the low activation energy for enzymatic substrate hydrolysis and the low thermostability of enzymes from cold-adapted organisms. Benefits can also be deduced from the frost hardiness and frost resistance of cold-adapted plants and animals.
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