We are very pleased to put forth the 'Laboratory Manual of Pharmacognosy and Phyto chemistry II'. This manual is prepared as per PCI B. Pharm course regulations 2014 and is divided into four sections i.e detection of crude drugs with morphology, histology, powder characteristics; isolation and detection of phyto constituents; isolation of phyto constituents by chromatography techniques and analysis of crude drugs by chemical tests. The traditional as well as modern methods used for identification of crude drugs, isolation methods of phyto constituents and their detection are included in this manual. This manual is designed for 'outcome-based education' and each experiment is arranged in a uniform way such as practical significance, practical outcomes (PrOs) and its mapping with course outcomes, theory, resources used, procedure, precautions, observations, result, conclusion, references, and synopsis questions. Each experiment offers an opportunity to perform practical work, allowing students to gain proficiency in effectively managing equipment, handling glassware, chemicals and staining agents/ reagents, and writing conclusion. In addition, questions are provided at the end of the experiments to enhance students' knowledge, which will be beneficial for them as they pursue higher studies. During the laboratory period you will have to multitask, while you are doing experiment. It is essential to document properly what you do and what you observe while doing the practical. Always plan your work ahead and think about what you are doing, why you are doing it, what is happening and what you can conclude from your experiment. We acknowledge the help and co-operation extended by various persons in bringing out this manual. We are highly indebted to the authors of various books and articles mentioned in the reference which became a major source of information for writing this manual. We also thank the publishers, designers and printers who graciously worked hard to publish this manual in time. We hope that this manual will assist students in understanding concepts, principles, and performing procedures. We wish you all the best!