Prem Jose Vazhacharickal et al.'s Books

Synthesis of nanoparticles (Ag, Cu and Zn) from Glycosmis pentaphylla, Macaranga peltata, Emilia sonchifolia, Tabernaemontana divericata and Clerodendrum infortunatum leaves extract

By: Prem Jose Vazhacharickal,Sruthi S Nair

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Synthesis of nanoparticles (Ag, Cu and Zn) from Glycosmis pentaphylla, Macaranga peltata, Emilia sonchifolia, Tabernaemontana divericata and Clerodendrum infortunatum leaves extract

By: Prem Jose Vazhacharickal,Sruthi S Nair

Nanoparticles are particles with dimension on the range 10-9 and 10-10. Green synthesis is the new method developed for the synthesis of nanoparticles which is small in size, large surface area and eco-friendly. In India plant and tree leafs are used as medicine for the treatment of various diseases. They are rich source of antimicrobial agents. Silver, copper and zinc nanoparticles are synthesised from the leaf extract of different plants. Leaf extracts of Glycosmis pentaphylla (Pannal), Macaranga peltata (Vatta), Emilia sonchifolia (Muyal cheviyan), Tabernaemontana divericata (Nandhiar vattam) and Clerodendrum infortunatum (Peravam) are used for the synthesis of nanoparticles. Leaf extract is added to the prepared stock solution of1mM silver nitrate, 100mM copper sulphate and100mM zinc sulphate. Synthesised nanoparticles were characterized by UV-VIS spectrophotometry to confirm the formation of nanoparticles. Anti-bacterial are used to treats bacterial infections. The leaf extract and silver nitrate, copper sulphate, zinc sulphate has antibacterial activity. The antibacterial activity of synthesised nanoparticle is determined using agar well diffusion method. The synthesised nanoparticles exhibit anti- bacterial activity by inhibiting the growth of gram negative and gram positive bacteria.

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ISBN 10

1983159476

ISBN 13

9781983159473

Synthesis of nanoparticles (Ag, Cu and Zn) from plant latex (Colocasia esculenta; Ficus exasperata; Hevea brasilliensis; Musa paradisiaca; Croton variegatum) and evaluation of antibacterial activity

By: Prem Jose Vazhacharickal,Meera Krishnan

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Synthesis of nanoparticles (Ag, Cu and Zn) from plant latex (Colocasia esculenta; Ficus exasperata; Hevea brasilliensis; Musa paradisiaca; Croton variegatum) and evaluation of antibacterial activity

By: Prem Jose Vazhacharickal,Meera Krishnan

Nanotechnology is the field of study of materials at nanoscale. It involves the production, manipulation, and use of materials ranging in size from less than one micron to that of individual atoms from not only chemical approaches but also biological materials. Silver, Copper and Zinc nanoparticles were successfully synthesised from Silver nitrate, Copper sulphate and Zinc sulphate respectively through a simple green and natural route using latex of 5 different plant taxa. Nanoparticle formation was proved by UV-vis spectroscopy. The antimicrobial well diffusion method used was give information about the antibacterial activity of latex nanoparticles towards 5 different bacterial species by measuring the zone of inhibition. The use of two dilutions of latex solution was used for the comparative study of zone of inhibition. As nanoparticles have great application in medical world like gene therapy, cancer therapy, drug delivery, etc. So medical world also accept the plant world for nanoparticle synthesis and mainly welcome the angiosperms for their potentiality of synthesis of non-polluted, environmentally acceptable, safety for human health nanoparticles.

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Prem Jose

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ISBN 10

1983169862

ISBN 13

9781983169861

Green synthesis of copper and zinc nanoparticles from plant extracts and evaluation of their antifungal activity against Fusarium oxysporum cubense: an overview

By: Prem Jose Vazhacharickal,Nissy Mary Joseph,Sajeshkumar N.K,Jiby John Mathew,P. E Sreejith,M Sabu

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Green synthesis of copper and zinc nanoparticles from plant extracts and evaluation of their antifungal activity against Fusarium oxysporum cubense: an overview

By: Prem Jose Vazhacharickal,Nissy Mary Joseph,Sajeshkumar N.K,Jiby John Mathew,P. E Sreejith,M Sabu

Soil borne diseases which are caused to various plants include a wide variety of soil microbes like fungi and bacteria, among which Fusarium wilt is one such disease caused by Fusarium oxysporum cubense in banana plants. Wilt disease or the panama disease of plant is among the most destructive disease of banana in the tropics and even the control methods like field sanitation, soil treatments and crop rotations have not been a long term control for this disease. An alternative method of treating Fusarium oxysporum was adopted by using various natural plant leaves of Chromolaena odorata, Justicia adhatoda, Glycosmis pentaphylla, Azadirachta indica, Gliricidia sepium, Piper nigrum, Ocimum tenuiflorum and Tabernaemontana divaricate. Nanoparticles are small particles with a dimension of 10-9 and 10-10. Green synthesis is a new method developed for the synthesis of nanoparticles which is small in size, large surface area and eco- friendly. Leaf extracts of these plants were used for synthesis of copper and zinc nanoparticles, as nanoparticles are powerful antimicrobial agents. The extract is prepared with a stock solution of 100mM copper sulphate and 100mM zinc sulphate. The leaf extracts were prepared with 5 solvents (Distilled water, Propane, Hexane, Acetone and Methanol). The action of plant leaves were observed by the zone of inhibition obtained with a concentration of 50, 100 and 150µl respectively. The result was more in copper nanoparticles of leaf extract as compared to the zinc nanoparticles of particular leaf extracts but the zinc particles with methanol and propane showed good result with particular leaves. In dried condition of leaves copper nanoparticles with propane as solvent exhibited a greater zone of inhibition. Moreover the solvent, methanol showed good results with both zinc and copper nanoparticles. The synthesized nanoparticle were characterized by UV-VIS spectrophotometry to confirm the formation of nanoparticles. Green synthesis is used namely because of low cost, simple, use of less toxic materials, most important is eco-friendly.

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141

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Amazon Publishers, USA

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ISBN 10

1095343858

ISBN 13

9781095343852

Evaluation of anti-microbial and anti-oxidant and phytochemical activity of Eupatorium triplinerve Vahl against wound infections

By: Prem Jose Vazhacharickal,John Joseph,Athira Vasanthan

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Evaluation of anti-microbial and anti-oxidant and phytochemical activity of Eupatorium triplinerve Vahl against wound infections

By: Prem Jose Vazhacharickal,John Joseph,Athira Vasanthan

Medicinal plants have bioactive compounds which are used for various human disease and also an important role in wound healing. The present study is to investigate about the Antimicrobial, phytochemical and antioxidant activity of Eupatorium triplinerve Vahl. The extracts are studied against human wound and burn pathogenic bacterial stains. E. coli, Klebsiella species, Pseudomonas species, Staphylococcus aureus, Enterobacter species. From the phytochemical study is shows the presence of steroids, glycosides, tannins, phenols, saponins and flavonoids. Plant extracts compounds were separated by thin layer chromatography and identification based on Rf values. The presence of vitamin C play and important role of antioxidants.

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ISBN 10

1717830021

ISBN 13

9781717830029

Morphological diversity of jackfruit (Artocarpus heterophyllus) varieties in Kerala

By: Prem Jose Vazhacharickal,Sajeshkumar N.K,Jiby John Mathew,Alen N Albin

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Morphological diversity of jackfruit (Artocarpus heterophyllus) varieties in Kerala

By: Prem Jose Vazhacharickal,Sajeshkumar N.K,Jiby John Mathew,Alen N Albin

Jackfruit (Artocarpus heterophyllus) is commonly grown in home gardens of tropical and sub-tropical countries. The fruit contains high levels of carbohydrates, protein, starch, calcium and vitamins. Jack fruit has diverse medicinal uses especially as anti-oxidant, anti-inflammatory, antimicrobial, anti-cancer and anti-fungal agent. Jackfruit is considered to be an underutilized fruit where most of the fruits get wasted due to ignorance, lack of post-harvest technology and gaps in supply chain systems. Jackfruit contains more protein, calcium, iron, vitamins and other essential nutrients, compared to common fruits. To find out the morphological variations among jackfruits in Kerala we conducted an elaborate survey and found out varieties using parameters such as tree characteristics, leaf characterises, fruit characteristics of selected samples as measure. Both qualitative and quantitative data were measured using field visit and standard methods and morphological indicators. After analysis of data morphological variations among the jack fruit samples were identified. This includes the morphological difference between fruit stalk leaf and branch leaves. In the fruits themselves, colour, shape and size are also found to vary among the samples. These variations may have some relationship to climatic factors such as rainfall, pH and soil properties. Morphological variations affect the selection of the varieties. Encouragement should be given to the marketing as well as creation of value added products from this underutilized fruit tree.

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ISBN 10

173146410X

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9781731464101

Antibacterial activity of the flower extracts of Caesalpinia pulcherrima L. against eye infection causing pathogens

By: Prem Jose Vazhacharickal,Noby Mathew,Athira T Rameshan

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Antibacterial activity of the flower extracts of Caesalpinia pulcherrima L. against eye infection causing pathogens

By: Prem Jose Vazhacharickal,Noby Mathew,Athira T Rameshan

Caesalpinia pulcherrima flowers have excellent antibacterial property. The present study aims to evaluate the antibacterial property Caesalpinia pulcherrima flower extract against clinically isolated eye infection causing Staphylococcus auerus. The (MIC) minimum inhibitory concentration and (MBC) minimum bactericidal concentration was found to be 200 µg/ml and 400 µg/ml. Antioxidant activity, spectroscopic and phytochemical analysis of the extract was also studied. This study concludes that Caesalpinia pulcherrima can be used as a potential natural remedy to treat eye infection.

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ISBN 10

197343735X

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9781973437352

Synthesis of silver, copper and zinc nanoparticles from Carica papaya, Annona muricata, Passiflora edulis, Cycas circinalis, Pouteria campechiana seeds and evaluation of their antibacterial activity

By: Prem Jose Vazhacharickal,Jismon Thomas

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Synthesis of silver, copper and zinc nanoparticles from Carica papaya, Annona muricata, Passiflora edulis, Cycas circinalis, Pouteria campechiana seeds and evaluation of their antibacterial activity

By: Prem Jose Vazhacharickal,Jismon Thomas

Nanotechnology is an emerging field of science. It has increased applications in diverse area for the development of new materials at nanoscale levels. Synthesis of nanoparticles using biological methods is referred as greener synthesis of nanoparticles. Seed extracts of papaya (Carica papaya), Mullatha (Annona muricata), Passion fruit (Passiflora edulis), Eenth (Cycas circinalis), Egg fruit (Pouteria campechiana) are used for the synthesis of silver, copper, and zinc nanoparticles. These plants have medicinal as well as antibacterial activity. Nanoparticles prepared from these seed extracts have antibacterial activity. Synthesized nanoparticles were characterized by UV-VIS Spectrophotometry. Silver nanoparticles shows maximum peak at 385 nm. Copper nanoparticles shows maximum peak at 680 nm. Zinc nanoparticles shows maximum peak at 350 nm. Synthesized silver, copper and zinc nanoparticles shows antibacterial activity against Salmonella species, Pseudomonas species, Staphylococcus species, E. coli and Klebsiella species. Antimicrobial assay was performed by agar well diffusion method using Muller Hinton agar media. when antibacterial activity of silver, copper and zinc nanoparticles from 3 different concentrations were observed, nanoparticles have 60 µl concentration shows maximum activity against these microbes. Silver nanoparticles shows greater antibacterial activity compared to silver nitrate and seed extract. Copper nanoparticles shows greater antibacterial activity compared to copper Sulphate and seed extract. Zinc nanoparticles shows greater antibacterial activity compared to zinc Sulphate and seed extract. Maximum zone of inhibition was at 60 µl for all the bacterial cultures. This green synthesis method is alternative to chemical method, since it is cheap, pollutant free and eco-friendly.

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Prem Jose

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ISBN 10

1983152307

ISBN 13

9781983152306

Evaluation of anti-microbial and anti-inflammatory action of castor and papaya seed extracts against Staphylococcus aureus

By: Prem Jose Vazhacharickal,John Joseph,Ravathy Krishna

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Evaluation of anti-microbial and anti-inflammatory action of castor and papaya seed extracts against Staphylococcus aureus

By: Prem Jose Vazhacharickal,John Joseph,Ravathy Krishna

The study was designed to investigate the antimicrobial and anti-inflammatory efficiency of aqueous extract of dried papaya and castor seeds, which are known to possess various therapeutic properties and economical applications. Microtiter Plate Assay method was used to find out antimicrobial analysis. The aqueous extract has a good antibacterial activity against Staphylococcus aureus and the inhibitory percentage for papaya and castor are 79.9% and 85% respectively with the maximum concentration used in this. The anti-inflammatory action is done by HRBC Membrane Stabilization Assay and papaya and castor seed extract showed 56% and 46% inhibition with the maximum concentration used in this. Thus the seeds can be suggested us the preventive agents in diseases and conditions like cancer, edema, tumour, diabetics, anti-viral, cardiovascular diseases, neuro- degenerative diseases to certain extent.

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Prem Jose

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ISBN 10

1717831672

ISBN 13

9781717831675

Isolation, characterization and optimization of dye degrading bacteria from natural source

An overview

By: Prem Jose Vazhacharickal,John Joseph,Jiby John Mathew,Sajeshkumar N.K.,Delmy Abraham

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Isolation, characterization and optimization of dye degrading bacteria from natural source

An overview

By: Prem Jose Vazhacharickal,John Joseph,Jiby John Mathew,Sajeshkumar N.K.,Delmy Abraham

Scientific Study from the year 2016 in the subject Biology - Micro- and Molecular Biology, grade: 1.5, Mar Augusthinose College, language: English, abstract: In this study an attempt was made to evaluate the colour degradation capabilities by collecting the contaminated soil sample from Kalady area and serial dilution was done upto 10-6. From the dilution 10-5 was taken and spread plated on Nutrient agar. From the above plate, isolated colonies was obtained which was found to be Bacillus sp and Pseudomonas sp respectively by morphological, microscopical and biochemical method. The isolated colonies was taken for degradation studies with 1% dye and 1% inoculum in Nutrient broth and OD values and colour change was noted. It was found to be Bacillus sp has more degrading capacity in yellow colour than Pseudmonas sp. The optimization studies was done with Bacillus sp having different concentration of colour (2, 4, 6) with varying pH (4,6,8) and temperature (37°C, 40°C and room temperature). The result was found to be having the concentration of colour with 4% having pH 4 and temperature 37°C.

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GRIN Verlag

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ISBN 10

3668478597

ISBN 13

9783668478596

Soil properties and enzymatic characterization of jackfruit (Artocarpus heterophyllus) varieties in Kerala

By: Dr. Prem Jose Vazhacharickal,Sajeshkumar N.K.,Jiby John Mathew

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Soil properties and enzymatic characterization of jackfruit (Artocarpus heterophyllus) varieties in Kerala

By: Dr. Prem Jose Vazhacharickal,Sajeshkumar N.K.,Jiby John Mathew

Scientific Study from the year 2016 in the subject Agrarian Studies, , language: English, abstract: Jackfruit (Artocarpus heterophyllus) is commonly grown in home gardens of tropical and sub-tropical countries. The fruit contains high levels of carbohydrates, protein, starch, calcium and vitamins. The Artocarpus heterophyllus is believed indigenous to the Western Ghats of India. It is adapted only to the humid tropical and near tropical climate. The tree flourishes in rich deep soil of medium or open texture, sometimes on deep gravelly or laterite soil. In India they say that the tree grows tall and thin on sand, short thick on sandy land. Here the physical and chemical properties of soil from surrounding plots of different varieties of Arthocarpus heterophyllus have been analysed by using different technical methods and evaluating the effects of soil property on jackfruit nutritional quality. It was observed that there was a considerable difference in case of some minerals likes phosphorous, potassium, magnesium, zinc and manganese. Change in the soil pH also noticed. The pH also plays a major role in the sweetness of the jackfruit. The optimum pH of the amylase inside the jackfruit was found to be 6.2 to 6.8. The result shows that the soils that have higher pH have more sweet flakes. Proximate nutritional composition of the flake reveals the presence of protein, fat and fibre in a greater amount in some flake and these flakes were sweeter than the others. There was a much difference in the sugar level also. Increased protein, fat and fibre content found in flake of the trees that were growing in soil that have higher concentration of minerals. Further studies such as molecular studies, enzymatic studies should conduct to identify the whole variation among jackfruit in Kerala. Encouragements should be done to the marketing as well as value added food products from this underutilized fruit tree.

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134

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GRIN Verlag

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ISBN 10

3668376565

ISBN 13

9783668376564

Biosorption of heavy metals by free and immobilized cells of Pseudomonas aeruginosa and Bacillus subtilis isolated from garden soils

By: Prem Jose Vazhacharickal,John Joseph,Soniya Mathew

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Biosorption of heavy metals by free and immobilized cells of Pseudomonas aeruginosa and Bacillus subtilis isolated from garden soils

By: Prem Jose Vazhacharickal,John Joseph,Soniya Mathew

Human populations need methods and technologies to clean waters and diminish the environmental dangers related to technological progress. Biosorption can be one such solution to clean up metal contamination. In the present study P.aeruginosa and B.subtilis was isolated from rhizosphere soil by serial dilution and plate count technique. Both free and immobilized form of isolates were tested for the biosorption of chromium (Cr), molybdenum (Mo), iron (Fe) and copper (Cu). Both the strains were able to remove metal from solutions. Free cells of B.subtilis and P.aeruginosa showed maximum iron removal 86.6% and 92.2% respectively. Free cells of B.subtilis is better in chromium removal (92%) that P.aeruginosa. and B.subtilis was equally effective in the removal of copper (98.1%). Maximum microorganism removal was observed with immobilized cells of B.subtilis; 87.5%. agitation increases the rate of biosorption when compared to static condition. The experiments were also conducted at 37°C and showed slight increase in the rate of biosorption. Microbial metal bioremediation is an efficient strategy due to its low cost, high efficiency and eco-friendly nature. This work summarize the potential of micros in metals remediation.

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Prem Jose

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ISBN 10

1980943540

ISBN 13

9781980943549

Nesting behavior and habitats of the stingless bee Trigona iridipennis Smith in Kerala

The current status of knowledge

By: Prem Jose Vazhacharickal,Sajan Jose K.

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Nesting behavior and habitats of the stingless bee Trigona iridipennis Smith in Kerala

The current status of knowledge

By: Prem Jose Vazhacharickal,Sajan Jose K.

Scientific Study from the year 2016 in the subject Biology - Miscellaneous, , language: English, abstract: Stingless bees are highly social insects which populated the tropical earth 65 million years ago and have been around much longer than honey bees. They are limited to the tropics and subtropics, lack a venom apparatus and cannot sting. Impacts of anthropogenic influences on honey bees have already been reported. Recent studies also showed that the nesting behaviour of Trigona iridipennis Smith in its natural habitat also varies due to interaction, pheromones and environmental stimulus. Little is reported so far about the various natural and domesticated nesting of the Trigona iridipennis Smith in Kerala. Based on thise background, our objectives of this study were to 1) to characterize the Meliponiculture 2) to identify the various natural habitats and domestication materials for nest construction and different types of nests used across Kerala. Various beekeeping methods preferred by farmers across Kerala for the cultivation of Trigona iridipennis Smith. Each nest has its own advantage and disadvantage. During the survey, the most preferred ones were wooden box. Even then according to the easy availability and production cost different nests like earthen pot, bamboo nodes, coconut shell, PVC pipes etc were used. The most preferred natural nesting sites by Trigona iridipennis Smith were mud/stone walls in our study. Being a social insect, Trigona iridipennis Smith shows great diversity in nesting pattern in natural habitats as well as anthropogenic habitats. The shift towards anthropogenic habitats than natural ones may be due to destruction of natural habitats as well as the availability of manmade habitats.

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GRIN Verlag

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ISBN 10

3668366233

ISBN 13

9783668366237

Swarming, drone movements, foraging behavior and associated plants of stingless bees (Trigona iridipennis Smith) in Kerala

An overview

By: Prem Jose Vazhacharickal,Sajan Jose K.

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Swarming, drone movements, foraging behavior and associated plants of stingless bees (Trigona iridipennis Smith) in Kerala

An overview

By: Prem Jose Vazhacharickal,Sajan Jose K.

Scientific Study from the year 2014 in the subject Biology - Zoology, , language: English, abstract: Stingless bees are highly social insects which populated the tropical earth 65 million years ago longer than honey bees. They are limited to tropics and subtropics lacking venom apparatus and cannot sting. Impacts of anthropogenic influences on honey bees were already reported. Based on these back ground, the objectives of this study were to 1) to characterize the swarming and drone movements of Trigona iridipennis Smith in Kerala 2) to identify the foraging behaviour, colour preference and various plants associated with Trigona iridipennis Smith for nest construction as well as dietary sources. Feral as well as hived colonies of stingless bees were located and fixed at different parts of Kerala for observations on their foraging activity. Bee traffic was also measured during various timings across a bright sunny day in November 2013 and between colonies. Bee traffic among Trigona iridipennis Smith varied widely among the selected four colonies during various time intervals. In all the colonies bee traffic starts between 6 to 7 am and end by 7 to 7.10 pm. Bee traffic exhibited two peaks. One during 10 to 10.10 am and the other 2 to 3.10 pm. But in colony 4 the picture is different, where the morning peak was 11 to 11.10 am and the afternoon peak was 3 to 3.10 pm. Trigona iridipennis Smith shows great diversity in plant selection for dietary as well as resin sources. The shift towards ornamental plants for foraging may be an adaptation evolved in response to human modification of the environment. The bees collect resin from a variety of sources for building nest, its maintenance and also for defence. Bee traffic is found to be related to time, season, and strength of the colony. The bees preferred white and yellow coloured flowers than pink and red. The study also highlights the various food sources of Trigona iridipennis Smith in Kerala which can be further explored for flourishing melliponiculture.

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GRIN Verlag

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ISBN 10

3668370699

ISBN 13

9783668370692

Antimicrobial and phytochemical analysis of lime juice and different types of honey. An overview

By: Prem Jose Vazhacharickal,Sajeshkumar N. K.,Jiby John Mathew,Steffy Maria Augustine

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Antimicrobial and phytochemical analysis of lime juice and different types of honey. An overview

By: Prem Jose Vazhacharickal,Sajeshkumar N. K.,Jiby John Mathew,Steffy Maria Augustine

Scientific Study from the year 2016 in the subject Biology - Micro- and Molecular Biology, grade: 1.5, Mar Augusthinose College, language: English, abstract: Citrus, one of the major genes of Rutaceae family and most economically important fruit tree and widely cultivated throughout the country. The Citrus have high nutritional value and medicinal value. Honey and lemon-honey are traditional remedies in the Middle East and China and for many centuries and have been used in the treatment and prevention of the common cold and various upper respiratory tract infections. Three types of honey were collected; ‘Cheruthen’-produced by bees belongs to the Trigona irridipennis species; Vanthen’- produced by bees belongs to the Apis indica species; ‘Kattutthen’- produced by bees belongs to the Apis dorsata species. The antibacterial activites of honey samples and lime juice were tested aganist Bacillus, Klebsiella, E.coli, Staphylococcus and Micrococcus. The result showed that the samples have differnt antimicrobial activity. Antimicrobial activity of Cheruthen against Klebsiella species showed a zone of inhibition of 10.1 ± 0.73 mm, when 100 μl of Cheruthen is applied. When 200 μl of Cheruthen is applied the zone of inhibition was 30.1 ± 0.23 mm. Antimicrobial activity of Cheruthen against E.coli showed a zone of inhibition of 10.1 ± 0.13 mm, when 100 μl of cheruthen is applied. When 200 μl of cheruthen is applied the zone of inhibition was 30.2 ± 0.23 mm. Also the phytochemical examination of lime juice and honey samples showed that different types of phytochemical substances are present in both lime juice and different types of honey samples. Further studies are required to reveal the role of each phytochemical and its contribution to the antimicrobial properties of the samples included in this study.

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GRIN Verlag

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3668476772

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9783668476776

Isolation and molecular characterization of dye tolerant bacteria from dye contaminated soil in Kerala

By: Prem Jose Vazhacharickal,Sajeshkumar N.K,Jiby John Mathew,Nimisha Vinod

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Isolation and molecular characterization of dye tolerant bacteria from dye contaminated soil in Kerala

By: Prem Jose Vazhacharickal,Sajeshkumar N.K,Jiby John Mathew,Nimisha Vinod

Scientific Study from the year 2017 in the subject Biology - Micro- and Molecular Biology, grade: 1.5, Mar Augusthinose College, language: English, abstract: The dyes are complex chemical compounds that imparts colour to substances. On the urge of urbanisation, the use of synthetic dyes is increasing largely and its untreated effluent release causes serious environmental pollution affecting water bodies by disturbing the aquatic ecosystem, soil, plants, animals, humans. The recalcitrant nature of these dyes limits its treatment using conventional methods wherein biological methods using microorganisms are reported to completely mineralize the dyes lowering the release of degradation products. The present work focuses on the biodegradation of synthetic dyes by isolating bacteria from a dyeing unit. Soil samples from the dye contaminated soils was collected, its degradation potentiality was observed using three major dye of studies congo red, brilliant green and methylene blue within 24 hr incubation. Maximum decoloarized dye (congo red and brilliant green) were chosen, serially diluted to 10-5 and plated to obtain two distinct colonies from decolorized congo red (CR1, CR2) and three distinct colonies from decolorized brilliant green (BG1, BG2, BG3). These isolates were biochemically characterized. Molecular characterization was performed by isolating DNA from five isolates and amplified it using PCR, with the 16s rRNA gene primer. The PCR amplification product having approximately 1500bp were sequenced, edited and searched using BLAST against the known sequences within NCBI databases. The isolates were identified to be CR1 as Pseudomonas (88% identity), CR2 as Aeromonas (89% identical) and BG1/BG2 were confirmed to belong to same genus as Bacillus (99% identical). The phylogenetic tree showed a clear divergence between isolated species. Furthermore, the dye tolerance of isolates were observed by optimization analysis to be as CR1 and CR2 tolerated up to 4% of congo red dye and among BG1, BG2 and BG3; BG1 tolerated up to 0.50% of brilliant green dye. Thus, CR1 and CR2 were observed to be potent azo dye decolourizers. Recombinant DNA Technology can be applied in this field that can make the above future application more reliable.

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GRIN Verlag

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ISBN 10

3668446873

ISBN 13

9783668446878

Cholesterol lowering effect of Tamarind (Tamarindus indica)

By: Prem Jose Vazhacharickal,Jiby John Mathew,Sajeshkumar N.K.,Berin Babu

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Cholesterol lowering effect of Tamarind (Tamarindus indica)

By: Prem Jose Vazhacharickal,Jiby John Mathew,Sajeshkumar N.K.,Berin Babu

Scientific Study from the year 2017 in the subject Chemistry - Bio-chemistry, grade: 1.5, Mar Augusthinose College, course: Biotechnology, language: English, abstract: Aqueous extract of the fruit pulp of Tamarind (Tamarindus indica) were evaluated for cholesterol lowering effect, in vitro, against various fatty food materials. People consume food items made by chicken, beef, mutton, egg and fish and it contains large amount of fat. This study aims to analyze the effect of Tamarindus indica in reducing the cholesterol level in this fat compound using water extract of the pulp. For this fatty food samples like egg yolk, pork fat, chicken fat, ghee and cod liver oil were treated with the extract and cholesterol level was estimated by Zak’s method for a period of time. Phytochemical constituents present in water extract of Tamarindus indica pulp includes Alkaloids, saponins, steroids, phlobatannins, carbohydrate, terpenoids, phenols, coumarins and leucoanthocyanins. The in vitro cholesterol lowering effect of Tamarindus indica pulp extract shows a positive result on chicken fat, ghee and egg yolk. But in case of pork fat and cod liver oil no beneficial change was observed.

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3668511675

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9783668511675

Book's by Prem Jose Vazhacharickal et al.

Synthesis of nanoparticles (Ag, Cu and Zn) from Glycosmis pentaphylla, Macaranga peltata, Emilia sonchifolia, Tabernaemontana divericata and Clerodendrum infortunatum leaves extract

Synthesis of nanoparticles (Ag, Cu and Zn) from Glycosmis pentaphylla, Macaranga peltata, Emilia sonchifolia, Tabernaemontana divericata and Clerodendrum infortunatum leaves extract

Synthesis of nanoparticles (Ag, Cu and Zn) from plant latex (Colocasia esculenta; Ficus exasperata; Hevea brasilliensis; Musa paradisiaca; Croton variegatum) and evaluation of antibacterial activity

Synthesis of nanoparticles (Ag, Cu and Zn) from plant latex (Colocasia esculenta; Ficus exasperata; Hevea brasilliensis; Musa paradisiaca; Croton variegatum) and evaluation of antibacterial activity

Green synthesis of copper and zinc nanoparticles from plant extracts and evaluation of their antifungal activity against Fusarium oxysporum cubense: an overview

Green synthesis of copper and zinc nanoparticles from plant extracts and evaluation of their antifungal activity against Fusarium oxysporum cubense: an overview

Evaluation of anti-microbial and anti-oxidant and phytochemical activity of Eupatorium triplinerve Vahl against wound infections

Evaluation of anti-microbial and anti-oxidant and phytochemical activity of Eupatorium triplinerve Vahl against wound infections

Morphological diversity of jackfruit (Artocarpus heterophyllus) varieties in Kerala

Morphological diversity of jackfruit (Artocarpus heterophyllus) varieties in Kerala

Antibacterial activity of the flower extracts of Caesalpinia pulcherrima L. against eye infection causing pathogens

Antibacterial activity of the flower extracts of Caesalpinia pulcherrima L. against eye infection causing pathogens

Synthesis of silver, copper and zinc nanoparticles from Carica papaya, Annona muricata, Passiflora edulis, Cycas circinalis, Pouteria campechiana seeds and evaluation of their antibacterial activity

Synthesis of silver, copper and zinc nanoparticles from Carica papaya, Annona muricata, Passiflora edulis, Cycas circinalis, Pouteria campechiana seeds and evaluation of their antibacterial activity

Evaluation of anti-microbial and anti-inflammatory action of castor and papaya seed extracts against Staphylococcus aureus

Evaluation of anti-microbial and anti-inflammatory action of castor and papaya seed extracts against Staphylococcus aureus

Isolation, characterization and optimization of dye degrading bacteria from natural source

Isolation, characterization and optimization of dye degrading bacteria from natural source

Soil properties and enzymatic characterization of jackfruit (Artocarpus heterophyllus) varieties in Kerala

Soil properties and enzymatic characterization of jackfruit (Artocarpus heterophyllus) varieties in Kerala

Biosorption of heavy metals by free and immobilized cells of Pseudomonas aeruginosa and Bacillus subtilis isolated from garden soils

Biosorption of heavy metals by free and immobilized cells of Pseudomonas aeruginosa and Bacillus subtilis isolated from garden soils

Nesting behavior and habitats of the stingless bee Trigona iridipennis Smith in Kerala

Nesting behavior and habitats of the stingless bee Trigona iridipennis Smith in Kerala

Swarming, drone movements, foraging behavior and associated plants of stingless bees (Trigona iridipennis Smith) in Kerala

Swarming, drone movements, foraging behavior and associated plants of stingless bees (Trigona iridipennis Smith) in Kerala

Antimicrobial and phytochemical analysis of lime juice and different types of honey. An overview

Antimicrobial and phytochemical analysis of lime juice and different types of honey. An overview

Isolation and molecular characterization of dye tolerant bacteria from dye contaminated soil in Kerala

Isolation and molecular characterization of dye tolerant bacteria from dye contaminated soil in Kerala

Cholesterol lowering effect of Tamarind (Tamarindus indica)

Cholesterol lowering effect of Tamarind (Tamarindus indica)

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