Food and nutrition security is one of the 17 Sustainable Development Goals enshrined in the 2030 Agenda for Sustainable Development. In an attempt to contribute to reaching this objective, school feeding programmes are serving meals to over 418 million pre-primary, primary and secondary schoolchildren around the world. The positive experience from a project supported by the Food and Agriculture Organization of the United Nations (FAO) in Angola, Honduras and Peru that incorporated locally procured fish into home-grown school feeding (HGSF) programmes led to the elaboration of this toolkit. This toolkit is designed to support governments, project designers, managers and practitioners involved in the fishery value chain and school feeding, who want to incorporate locally procured, safe, nutritious and affordable fish and fish products into their existing HGSFs. Therefore, this toolkit is expected to assist them during the rapid assessment of the situation of the school feeding and fishery sector, and the identification of challenges and opportunities present while incorporating fish and fish products into HGSFs. To this end, this toolkit adopts three main approaches: the Sustainable Food Value Chain for Nutrition to enhance the consideration of nutrition lens in the value chain approach; the gender-transformative approach to support women fisherfolk in their activities and increase their participation in school feeding programmes; and local and inclusive food procurement to connect public demand for food to small-scale fisherfolk. Specifically, this toolkit proposes 4 phases and 15 flexible and adaptable tools to sustainably serve fish and fish products at schools.