Quantum physics is based on four fundamental interactions of electromagnetic, weak, gravitational and strong forces. All the interactions are expressed in terms of fermion and boson fields which can describe the quantum states of electrons, nucleons and photons in atoms or nuclei. Correct behaviors of these particles can now be described by the basic field theory terminology, and this textbook explains, for the first time, quantum field theory in a unifying method. At present, modern quantum theory is at a critical junction between different theories, and this textbook presents a clear description of fundamental quantum fields with a sound theoretical framework. No exotic theoretical concepts such as general relativity nor spontaneous symmetry breaking nor quantum anomaly are adopted in this textbook, and indeed all the observed physical quantities can be well understood within the standard field theory framework without introducing any non-physical particles or fields. From this textbook, readers will be guided through a concrete future direction of quantum field theory and will learn how the motion of electrons in any kind of material can be understood in terms of fields or state vectors. Readers will also learn about application of basic field theory in quantum chemistry, quantum biology and so on. Fundamental Problems in Quantum Field Theory is a handy resource for undergraduate and graduate students as well as supervisors involved in advanced courses in quantum physics.
In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery. The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.
Quantum physics is based on four fundamental interactions of electromagnetic, weak, gravitational and strong forces. All the interactions are expressed in terms of fermion and boson fields which can describe the quantum states of electrons, nucleons and photons in atoms or nuclei. Correct behaviors of these particles can now be described by the basic field theory terminology, and this textbook explains, for the first time, quantum field theory in a unifying method. At present, modern quantum theory is at a critical junction between different theories, and this textbook presents a clear description of fundamental quantum fields with a sound theoretical framework. No exotic theoretical concepts such as general relativity nor spontaneous symmetry breaking nor quantum anomaly are adopted in this textbook, and indeed all the observed physical quantities can be well understood within the standard field theory framework without introducing any non-physical particles or fields. From this textbook, readers will be guided through a concrete future direction of quantum field theory and will learn how the motion of electrons in any kind of material can be understood in terms of fields or state vectors. Readers will also learn about application of basic field theory in quantum chemistry, quantum biology and so on. Fundamental Problems in Quantum Field Theory is a handy resource for undergraduate and graduate students as well as supervisors involved in advanced courses in quantum physics.
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