Masayoshi Nakasako's Books

Hydration Structures of Proteins

Atomic Details

By: Masayoshi Nakasako

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Hydration Structures of Proteins

Atomic Details

By: Masayoshi Nakasako

This book describes hydration structures of proteins by combining experimental results with theoretical considerations. It is designed to introduce graduate students and researchers to microscopic views of the interactions between water and biological macromolecules and to provide them with an overview of the field. Topics on protein hydration from the past 25 years are examined, most of which involve crystallography, fluorescence measurements, and molecular dynamics simulations. In X-ray crystallography and molecular dynamics simulations, recent advances have accelerated the study of hydration structures over the entire surface of proteins. Experimentally, crystal structure analysis at cryogenic temperatures is advantageous in terms of visualizing the positions of hydration water molecules on the surfaces of proteins in their frozen-hydrated crystals. A set of massive data regarding hydration sites on protein surfaces provides an appropriate basis, enabling us to identify statistically significant trends in geometrical characteristics. Trajectories obtained from molecular dynamics simulations illustrate the motion of water molecules in the vicinity of protein surfaces at sufficiently high spatial and temporal resolution to study the influences of hydration on protein motion. Together with the results and implications of these studies, the physical principles of the measurement and simulation of protein hydration are briefly summarized at an undergraduate level. Further, the author presents recent results from statistical approaches to characterizing hydrogen-bond geometry in local hydration structures of proteins. The book equips readers to better understand the structures and modes of interaction at the interface between water and proteins. Referred to as “hydration structures”, they are the subject of much discussion, as they may help to answer the question of why water is indispensable for life at the molecular and atomic level.

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321

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Springer Nature

Published Date

ISBN 10

4431569197

ISBN 13

9784431569190

X-Ray Diffraction Imaging of Biological Cells

By: Masayoshi Nakasako

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X-Ray Diffraction Imaging of Biological Cells

By: Masayoshi Nakasako

In this book, the author describes the development of the experimental diffraction setup and structural analysis of non-crystalline particles from material science and biology. Recent advances in X-ray free electron laser (XFEL)-coherent X-ray diffraction imaging (CXDI) experiments allow for the structural analysis of non-crystalline particles to a resolution of 7 nm, and to a resolution of 20 nm for biological materials. Now XFEL-CXDI marks the dawn of a new era in structural analys of non-crystalline particles with dimensions larger than 100 nm, which was quite impossible in the 20th century. To conduct CXDI experiments in both synchrotron and XFEL facilities, the author has developed apparatuses, named KOTOBUKI-1 and TAKASAGO-6 for cryogenic diffraction experiments on frozen-hydrated non-crystalline particles at around 66 K. At the synchrotron facility, cryogenic diffraction experiments dramatically reduce radiation damage of specimen particles and allow tomography CXDI experiments. In addition, in XFEL experiments, non-crystalline particles scattered on thin support membranes and flash-cooled can be used to efficiently increase the rate of XFEL pulses. The rate, which depends on the number density of scattered particles and the size of X-ray beams, is currently 20-90%, probably the world record in XFEL-CXDI experiments. The experiment setups and results are introduced in this book. The author has also developed software suitable for efficiently processing of diffraction patterns and retrieving electron density maps of specimen particles based on the diffraction theory used in CXDI.

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243

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Springer

Published Date

ISBN 10

443156618X

ISBN 13

9784431566182

X-Ray Diffraction Imaging of Biological Cells

By: Masayoshi Nakasako

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X-Ray Diffraction Imaging of Biological Cells

By: Masayoshi Nakasako

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243

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Springer

Published Date

ISBN 10

443156618X

ISBN 13

9784431566182

Hydration Structures of Proteins

Atomic Details

By: Masayoshi Nakasako

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Hydration Structures of Proteins

Atomic Details

By: Masayoshi Nakasako

Average ratings

N/A

Write a review

Solve jigsaw puzzle

Age

Page Count

321

Publisher

Springer Nature

Published Date

ISBN 10

4431569197

ISBN 13

9784431569190

Book's by Masayoshi Nakasako

Hydration Structures of Proteins

Hydration Structures of Proteins

X-Ray Diffraction Imaging of Biological Cells

X-Ray Diffraction Imaging of Biological Cells

X-Ray Diffraction Imaging of Biological Cells

X-Ray Diffraction Imaging of Biological Cells

Hydration Structures of Proteins

Hydration Structures of Proteins

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