The period between 1950 and 1980 were the golden unique insights into how pathological processes affect years of transmission electron microscopy and produced cell organization. a plethora of new information on the structure of cells This information is vital to current work in which that was coupled to and followed by biochemical and the emphasis is on integrating approaches from functional studies. TEM was king and each micrograph proteomics, molecular biology, genetics, genomics, of a new object produced new information that led to molecular imaging and physiology and pathology to novel insights on cell and tissue organization and their understand cell functions and derangements in disease. functions. The quality of data represented by the images In this current era, there is a growing tendency to of cell and tissues had been perfected to a very high level substitut e modern light microscopic techniques for by the great microscopists of that era including Palade, electron microscopy, because it is less technically Porter, Fawcett, Sjostrand, Rhodin and many others. At demanding and is more readily available to researchers- present, the images that we see in leading journals for This atlas reminds us that the information obtained by the most part do not reach the same technical level and electron microscopy is invaluable and has no substitute.
This book gives a survey of the architecture of the Golgi apparatus, as revealed by morphological and cytochemical studies with a variety of cell types. The results presented include demonstrations of Golgi architecture in the course of cell differentiation, at varying functional cellular states, and under the influence of microtubule-disrupting agents. Emphasis is particularly placed on the organization of subsections of the Golgi apparatus and the questions of how Golgi subsections may be related to functional subcompartments of the Golgi system. By means of affinity-cytochemical approaches, using a palette of lectins of diverse sugar specificities, it is shown that functional subcompartments can be distributed in the complex Golgi system irrespective of the morphological subdivision in cis-medial-trans-transmost subsections. The use of pre- and post-embedment lectin-cytochemical approaches as a tool for the localization of functional Golgi subcompartments is of particular interest, especially as some of the lectins have been used in these approaches for the first time. This book intends to provide synoptic information on the architecture of the Golgi apparatus, its wide variability and possible arrangements of Golgi subcompartments.
The period between 1950 and 1980 were the golden unique insights into how pathological processes affect years of transmission electron microscopy and produced cell organization. a plethora of new information on the structure of cells This information is vital to current work in which that was coupled to and followed by biochemical and the emphasis is on integrating approaches from functional studies. TEM was king and each micrograph proteomics, molecular biology, genetics, genomics, of a new object produced new information that led to molecular imaging and physiology and pathology to novel insights on cell and tissue organization and their understand cell functions and derangements in disease. functions. The quality of data represented by the images In this current era, there is a growing tendency to of cell and tissues had been perfected to a very high level substitut e modern light microscopic techniques for by the great microscopists of that era including Palade, electron microscopy, because it is less technically Porter, Fawcett, Sjostrand, Rhodin and many others. At demanding and is more readily available to researchers- present, the images that we see in leading journals for This atlas reminds us that the information obtained by the most part do not reach the same technical level and electron microscopy is invaluable and has no substitute.
The period between 1950 and 1980 were the golden It also provides unique insights into how pathological years of transmission electron microscopy and pro- processes affect cell organization. duced a plethora of new information on the structure of This information is vital to current work in which cells that was coupled to and followed by biochemical the emphasis is on integrating approaches from p- and functional studies. TEM was king and each micro- teomics, molecular biology, molecular imaging and graph of a new object produced new information that physiology, and pathology to understand cell functions led to new insights on cell and tissue organization and and derangements in disease. In this current era, there is their functions. The quality of data represented by the a growing tendency to substitute modern light mic- images of cells and tissues had been perfected to a very scopic techniques for electron microscopy because it is high level by the great microscopists of the era including less technically demanding and is more readily available Palade, Porter, Fawcett, Sjostrand, Rhodin and many to researchers. This atlas reminds us that the infor- others. At present, the images that we see in leading tion obtained by electron microscopy is invaluable and journals for the most part do not reach the same techni- has no substitute.
This book gives a survey of the architecture of the Golgi apparatus, as revealed by morphological and cytochemical studies with a variety of cell types. The results presented include demonstrations of Golgi architecture in the course of cell differentiation, at varying functional cellular states, and under the influence of microtubule-disrupting agents. Emphasis is particularly placed on the organization of subsections of the Golgi apparatus and the questions of how Golgi subsections may be related to functional subcompartments of the Golgi system. By means of affinity-cytochemical approaches, using a palette of lectins of diverse sugar specificities, it is shown that functional subcompartments can be distributed in the complex Golgi system irrespective of the morphological subdivision in cis-medial-trans-transmost subsections. The use of pre- and post-embedment lectin-cytochemical approaches as a tool for the localization of functional Golgi subcompartments is of particular interest, especially as some of the lectins have been used in these approaches for the first time. This book intends to provide synoptic information on the architecture of the Golgi apparatus, its wide variability and possible arrangements of Golgi subcompartments.
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