In the last decade DNA sequencing costs have decreased over a magnitude, largely because of increasing throughput by incremental advances in tools, technologies and process improvements. Further cost reductions in this and in related proteomics technologies are expected as a result of the development of new high-throughput techniques and the computational machinery needed to analyze data generated. Automation in Proteomics & Genomics: An Engineering Case-Based Approach describes the automation technology currently in the areas of analysis, design, and integration, as well as providing basic biology concepts behind proteomics and genomics. The book also discusses the current technological limitations that can be viewed as an emerging market rather than a research bottleneck. Topics covered include: molecular biology fundamentals: from ‘blueprint’ (DNA) to ‘task list’ (RNA) to ‘molecular machine’ (protein); proteomics methods and technologies; modelling protein networks and interactions analysis via automation: DNA sequencing; microarrays and other parallelization technologies; protein characterization and identification; protein interaction and gene regulatory networks design via automation: DNA synthesis; RNA by design; building protein libraries; synthetic networks integration: multiple modalities; computational and experimental methods; trends in automation for genomics and proteomics new enabling technologies and future applications Automation in Proteomics & Genomics: An Engineering Case-Based Approach is an essential guide to the current capabilities and challenges of high-throughput analysis of genes and proteins for bioinformaticians, engineers, chemists, and biologists interested in developing a cross-discipline problem-solving based approach to systems biology.
Squeezing ground represents a challenging operating environment as it may slow down or obstruct TBM operation. Due to the geometrical constraints of the equipment, relatively small convergences of 10-20 cm may lead to considerable difficulties in the machine area (sticking of the cutter head, jamming of the shield) or in the back-up area (e.g., jamming of the back-up equipment, inadmissible convergences of the bored profile, damage to the tunnel support). Depending on the number and the length of the critical stretches, squeezing conditions may even call into question the feasibility of a TBM drive. On account of this, and bearing in mind the steady increase in the number of tunnels excavated with TBMs through so-called "difficult ground conditions", the topic investigated in this PhD thesis is of great practical relevance.Based upon case histories reported in the literature, Part I sets out firstly to give an overview of the specific problems of TBM tunnelling under squeezing conditions. Part II presents a computational model which simulates accurately and efficiently the advancing TBM and the installed tunnel support in one single computational step applying the so-called "steady state method". Part III advances a number of theory-based decision aids, which will support rapid, initial assessments to be made of thrust force requirements. Part IV investigates the complex problem of the interaction between the advancing TBM, the consolidating ground and the lining. Emphasis is thereby placed on the effect of the gross advance rate and the effect of ground permeability on shield loading during regular TBM operation (the boring process including short standstills) and during a long standstill.
Male genital disorders represent a common issue in medical practice, especially in the dermatological setting. Correct clinical evaluation of these disorders is essential when addressing the diagnosis, which in some cases may require histopathological confirmation. Depending on the disease, early diagnosis may be not only lifesaving, but also of fundamental importance to the planning of successful treatment. This atlas introduces the most common penile diseases, along with more rarely encountered ones. It provides invaluable guidance on clinical diagnosis by highlighting prominent clinical features and presenting particular videodermatoscopy findings when these are indicative of the diagnosis. In addition, for each condition the most appropriate treatment is proposed, taking into account recent therapeutic advances of proven benefit.
This issue of Immunology and Allergy Clinics provides the latest essential updates in angioedema. This potentially life-threatening condition, as is the case with laryngeal angioedema, is a rare but important disease. Leading physicians review the spectrum of angioedema to provide a framework for approaching the management of these patients. Differential diagnosis is discussed in depth, as a variety of other conditions can mimic some aspects of angioedema. Angioedema occurs both in conjunction with or separately from the more common swelling disorder, urticaria. Various types of angioedema that classically occur in the absence of urticaria are discussed. Multiple types of hereditary angioedema, idiopathic recurring angioedema, and Gleich’s Syndrome are covered.
In the last decade DNA sequencing costs have decreased over a magnitude, largely because of increasing throughput by incremental advances in tools, technologies and process improvements. Further cost reductions in this and in related proteomics technologies are expected as a result of the development of new high-throughput techniques and the computational machinery needed to analyze data generated. Automation in Proteomics & Genomics: An Engineering Case-Based Approach describes the automation technology currently in the areas of analysis, design, and integration, as well as providing basic biology concepts behind proteomics and genomics. The book also discusses the current technological limitations that can be viewed as an emerging market rather than a research bottleneck. Topics covered include: molecular biology fundamentals: from ‘blueprint’ (DNA) to ‘task list’ (RNA) to ‘molecular machine’ (protein); proteomics methods and technologies; modelling protein networks and interactions analysis via automation: DNA sequencing; microarrays and other parallelization technologies; protein characterization and identification; protein interaction and gene regulatory networks design via automation: DNA synthesis; RNA by design; building protein libraries; synthetic networks integration: multiple modalities; computational and experimental methods; trends in automation for genomics and proteomics new enabling technologies and future applications Automation in Proteomics & Genomics: An Engineering Case-Based Approach is an essential guide to the current capabilities and challenges of high-throughput analysis of genes and proteins for bioinformaticians, engineers, chemists, and biologists interested in developing a cross-discipline problem-solving based approach to systems biology.
Squeezing ground represents a challenging operating environment as it may slow down or obstruct TBM operation. Due to the geometrical constraints of the equipment, relatively small convergences of 10-20 cm may lead to considerable difficulties in the machine area (sticking of the cutter head, jamming of the shield) or in the back-up area (e.g., jamming of the back-up equipment, inadmissible convergences of the bored profile, damage to the tunnel support). Depending on the number and the length of the critical stretches, squeezing conditions may even call into question the feasibility of a TBM drive. On account of this, and bearing in mind the steady increase in the number of tunnels excavated with TBMs through so-called "difficult ground conditions", the topic investigated in this PhD thesis is of great practical relevance.Based upon case histories reported in the literature, Part I sets out firstly to give an overview of the specific problems of TBM tunnelling under squeezing conditions. Part II presents a computational model which simulates accurately and efficiently the advancing TBM and the installed tunnel support in one single computational step applying the so-called "steady state method". Part III advances a number of theory-based decision aids, which will support rapid, initial assessments to be made of thrust force requirements. Part IV investigates the complex problem of the interaction between the advancing TBM, the consolidating ground and the lining. Emphasis is thereby placed on the effect of the gross advance rate and the effect of ground permeability on shield loading during regular TBM operation (the boring process including short standstills) and during a long standstill.
Powerful engineering tools can help solve today's complex biological and biomedical research challenges - and this first-of-its-kind guide is paving the way . This trail-blazing work gives engineers a quantitative systems approach to bioinformatics research using computational tools drawn from technical disciplines. It presents biological processes in an engineering context to help engineers use their technical skills in solving novel biological problems and also to facilitate reverse engineering from biology in developing synthetic biological devices.
This trailblazing guide gives biological and biomedical research engineers a quantitative systems approach to bioinformatics research using computational tools drawn from technical disciplines. A major milestone in systems biology, this groundbreaking work points engineers to new frontiers in the convergence of engineering and biological research.
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