Linda C. Smith's Books

On Wings of Infinity

By: Linda C. Smith

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Infinity. It's something that never ends. There is no way to begin to imagine what that really means, but the Holy Spirit is infinite. On Wings of Infinity tells how by the leading of the Holy Spirit, I was able to experience the hand of God moving in my life and in my sons' lives in a mighty way. It is about the all-seeing and all-knowing God who hears you when you pray. It's about times when he created miracles for each one of my sons, individually, that saved their lives. Each incident brought me closer and closer to the realization that God really does care about everything in my life. Sometimes people think that knowing the God who created the universe would be impossible. But it is not. Actually, it is very simple. This is what I believe. You can spend your whole life, even as a child of God, never really understanding or knowing the voice of God. But, as a child of God, his spirit lives in you. And you can learn to know when he is speaking to you. You can really become aware that God wants to speak to you, to help you. He never forces himself on you. You have to desire, to want to hear him. The first part of James 4:8 (KJV) says, "Draw nigh to God, and he will draw nigh to you." When you begin to have moments of knowing that it is the Holy Spirit of God speaking to you, it becomes so precious. In this book, I will tell you of times that I learned how much I needed God to help me and the ways that he did. Most of them are complete miracles.

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100

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Christian Faith Publishing, Inc.

Published Date

ISBN 13

9798886165364

Technical Services Management, 1965-1990

A Quarter Century of Change and a Look to the Future

By: Ruth C Carter,Linda C Smith

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Technical Services Management, 1965-1990

A Quarter Century of Change and a Look to the Future

By: Ruth C Carter,Linda C Smith

Gain an in-depth understanding of changes in technical services that have taken place over a quarter century and look at future trends and changes that may occur. Technical Services Management surveys and analyzes technical services in libraries from 1965 to 1990, a formative period and one of great change in library operations. The book also identifies trends that continue to impact technical services operations in libraries today. Readers gain a comprehensive knowledge of where the field has been and where it is now to help them plan and prepare more effectively for the future. Most chapters are historical, combined with a firm grasp of the present and a glimpse or more at the future. They are grouped to reflect the various aspects of technical services. Trends in technical services are considered in chapters on the development of technical services literature and the major changes in technical services in school libraries. Chapters on the major subdivisions within technical services--acquisitions and collection development, cataloging, and preservation--trace changes in library operations and the impact of automation. Issues in catalog design are explored in chapters on the emergence of online public access catalogs, bibliographic utilities, and approaches to authority control. Efforts to improve subject access are addressed through chapters on subject cataloging, the Dewey Decimal Classification, and indexing in the U.S. and Great Britain. To keep pace with changes in technical services, changes in professional education and development are needed as documented in chapters on cataloging education, continuing education in technical services, and the role of professional organizations. The final chapter outlines new challenges in the future and new roles for librarians in an electronic environment. Effective planning for the future includes learning about the past. Technical Services Management, 1965--1990 is a vital resource for library historians, library educators, technical services librarians, and graduate students in library and information science who need to know “how things were” in order to see more clearly “how things will be.”

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370

Publisher

Routledge

Published Date

ISBN 10

1136590897

ISBN 13

9781136590894

To Advance the Race

Black Women's Higher Education from the Antebellum Era to the 1960s

By: Linda M. Perkins

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To Advance the Race

Black Women's Higher Education from the Antebellum Era to the 1960s

By: Linda M. Perkins

From the United States' earliest days, African Americans considered education essential for their freedom and progress. Linda M. Perkins’s study ranges across educational and geographical settings to tell the stories of Black women and girls as students, professors, and administrators. Beginning with early efforts and the establishment of abolitionist colleges, Perkins follows the history of Black women's post–Civil War experiences at elite white schools and public universities in northern and midwestern states. Their presence in Black institutions like Howard University marked another advancement, as did Black women becoming professors and administrators. But such progress intersected with race and education in the postwar era. As gender questions sparked conflict between educated Black women and Black men, it forced the former to contend with traditional notions of women’s roles even as the 1960s opened educational opportunities for all African Americans. A first of its kind history, To Advance the Race is an enlightening look at African American women and their multi-generational commitment to the ideal of education as a collective achievement.

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311

Publisher

University of Illinois Press

Published Date

ISBN 10

0252056590

ISBN 13

9780252056598

Five Ways of Doing Qualitative Analysis

Phenomenological Psychology, Grounded Theory, Discourse Analysis, Narrative Research, and Intuitive Inquiry

By: Kathy Charmaz,Linda M. McMullen

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Five Ways of Doing Qualitative Analysis

Phenomenological Psychology, Grounded Theory, Discourse Analysis, Narrative Research, and Intuitive Inquiry

By: Kathy Charmaz,Linda M. McMullen

This unique text provides a broad introduction to qualitative analysis together with concrete demonstrations and comparisons of five major approaches. Leading scholars apply their respective analytic lenses to a narrative account and interview featuring "Teresa," a young opera singer who experienced a career-changing illness. The resulting analyses vividly exemplify what each approach looks like in action. The researchers then probe the similarities and differences among their approaches; their distinctive purposes and strengths; the role, style, and subjectivity of the individual researcher; and the scientific and ethical complexities of conducting qualitative research. Also included are the research participant's responses to each analysis of her experience. A narrative account from another research participant, "Gail," can be used by readers to practice the kinds of analysis explored in the book.

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450

Publisher

Guilford Press

Published Date

ISBN 10

1609181425

ISBN 13

9781609181420

Progress in Drug Research

By: Hao Wu,Eric J. Lien,Linda L. Lien,Richard M. Schultz,Vishnu Ji Ram,Esteban Domingo,Paul Spence,Satya P. Gupta,Suraj P. Bhat,Elcira C. Villarreal

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Progress in Drug Research

By: Hao Wu,Eric J. Lien,Linda L. Lien,Richard M. Schultz,Vishnu Ji Ram,Esteban Domingo,Paul Spence,Satya P. Gupta,Suraj P. Bhat,Elcira C. Villarreal

Progress in Drug Research is a prestigious book series which provides extensive expert-written reviews on a wide spectrum of highly topical areas in current pharmaceutical and pharmacological research. It serves as an important source of information for researchers concerned with drug research and all those who need to keep abreast of the many recent developments in the quest for new and better medicines.

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373

Publisher

Birkhäuser

Published Date

ISBN 10

303488012X

ISBN 13

9783034880121

Women and AIDS

Negotiating Safer Practices, Care, and Representation

By: Ellen Cole,Esther D Rothblum,Linda K Fuller,Nancy Roth

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Women and AIDS

Negotiating Safer Practices, Care, and Representation

By: Ellen Cole,Esther D Rothblum,Linda K Fuller,Nancy Roth

For many women, the advice “Use a condom!” is not enough to help protect them from HIV infection. As Women and AIDS reveals, “negotiating” safer sex practices is a very complex issue for women who are involved in relationships where they do not enjoy physical, social, or economic equality. The book’s authors maintain that the key to curbing the spread of HIV and to caring for those already infected--is communication. Women and AIDS is the first volume to address HIV/AIDS and women from a communication perspective. This helpful guidebook addresses how women might achieve safer sexual and drug injection practices with partners, but it also explores women’s negotiation of the health care system as patients, medical research subjects, and caregivers. It challenges traditional assumptions about the relationship between care providers and patients and the meaning of patient compliance and raises important questions about gender, race, and class that are exacerbated by the epidemic. Designed to ground interventions in the realities of women’s lives, Women and AIDS discusses what women can do to get around communication and health care obstacles. To this end, you will learn about: using the media for HIV-related social action and to promote women’s views of HIV and sexuality prison health care for HIV-positive women cultural constructions of sex and drug sharing in a variety of communities long-term changes that will empower women delivering an HIV-positive diagnosis to patients gender roles and caregiving the language we use to talk about “Third World” women and “Asian AIDS” women AIDS filmmakers/videographers For the benefit of AIDS activists, health care providers, and counselors, Women and AIDS discusses women and their communication and awareness from virtually every angle. This book analyzes situations where communication breaks down--from the woman who can’t openly discuss safe sex with her partner, to the drunk college student who “hooks up,” to the doctor who gives an HIV-positive diagnosis without compassion--and offers communication solutions. This will help women avoid such risks, establish communication and safety in their lives, and construct meaningful roles in relationship to HIV/AIDS.

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346

Publisher

Routledge

Published Date

ISBN 10

1317712439

ISBN 13

9781317712435

Doing the Work of Reference

Practical Tips for Excelling as a Reference Librarian

By: Linda S Katz

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Doing the Work of Reference

Practical Tips for Excelling as a Reference Librarian

By: Linda S Katz

Become more versatile, competent, and resourceful with these practical suggestions! Becoming a first-class reference librarian demands proficiency in a wide range of skills. Doing the Work of Reference offers sound advice for the full spectrum of your responsibilities. Though many aspects of a reference librarian's work are changing with astonishing speed, the classic principles in this volume will never go out of date. This comprehensive volume begins with hints for orienting yourself to a new job and concludes with ideas for serving the profession. On the way, Doing the Work of Reference covers such diverse topics as working with student assistants, offering reference services to remote users, and keeping up your professional development. In addition, you will find strategies for dealing with technological change--not high-tech information that will become obsolete before the ink is dry, but ways of approaching the process of change that will work today, next week, and ten years from now. Doing the Work of Reference will help you increase your competence in: getting along with other staff members marketing the library to users and faculty handling ephemeral materials keeping students’attention in library instruction courses maintaining good relations with faculty increasing your subject knowledge and much more! This comprehensive guide is an essential handbook for librarians in the trenches. Whether you are a new librarian or a veteran at the reference desk, Doing the Work of Reference will help you burnish your skills.

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410

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Routledge

Published Date

ISBN 10

1317951891

ISBN 13

9781317951896

Priorities in Critical Care Nursing

By: Linda D. Urden,Kathleen M. Stacy,Mary E. Lough

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Priorities in Critical Care Nursing

By: Linda D. Urden,Kathleen M. Stacy,Mary E. Lough

Ensure you are up to date on all the common and urgent issues in the critical care unit with Priorities in Critical Care Nursing, 7th Edition! With its succinct coverage of all core critical care nursing topics, this evidence-based text is the perfect resource for both practicing nurses and nursing students alike. Using the latest, most authoritative research, this book will help you identify priorities to accurately and effectively manage patient care. Content spans the areas of medication, patient safety, patient education, nursing diagnosis, and collaborative management and much more to equip you for success in all aspects of critical care nursing. This new edition also features new case studies, new QSEN-focused call-out boxes throughout the text, a complete digital glossary, and revised chapter summaries. Evidence-based approach offers the most accurate and timely patient care recommendations based on the latest and most authoritative research, meta-analyses, and systematic reviews available. UNIQUE! Nursing Diagnosis Priorities boxes list the most urgent potential nursing diagnoses, with a page reference to the corresponding Nursing Management Plan. Nursing Management Plans provide a complete care plan for every Priority Diagnosis that includes the diagnosis, definition, defining characteristics, outcome criteria, nursing interventions, and rationales. Case studies with critical thinking questions test your understanding of key concepts and their practical applications. Concept maps help students understand common critical health conditions, including acute coronary syndrome, acute renal failure, ischemic stroke, and shock. Collaborative Management boxes guide you through the management of a wide variety of disorders. Patient Education boxes list the concepts that must be taught to the patient and the family before discharge from the ICU. Priority Medication boxes offer a foundation in the pharmacology used most in critical care. NEW! QSEN Evidence-Based Practice boxes use the PICOT framework to cover a timely topic and the research that underlies current patient care. NEW! TEACH for Nurses manual includes unique case studies, outlines, instructor resources, student resources, answer keys, and more. NEW! PowerPoint slides with unfolding case studies have been updated to include interactive questions and sample handoff information in the ISBARR format for appropriate chapters. NEW! Cultural Competency boxes provide information on basic cultural topics, including what cues to watch for and how to better provide culturally competent care. NEW! QSEN Teamwork and Collaboration boxes offer concise guidelines for effective handoffs, assessments, and communications between nurses and other hospital staff. NEW! QSEN Patient Safety Alert boxes highlight important guidelines and tips to ensure patient safety. NEW! QSEN Internet Resources boxes identify key organizations and websites for both general critical care practice and for each specific body system. NEW! Key points at the end of each chapter offer a quick study tool for students. NEW! More-detailed objectives now include every disorder covered in the chapter. NEW! Digital glossary on the Evolve companion site help to increase students' critical care nursing vocabulary.

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659

Publisher

Elsevier Health Sciences

Published Date

ISBN 10

0323320856

ISBN 13

9780323320856

Medical-Surgical Nursing - E-Book

By: Donna D. Ignatavicius,M. Linda Workman,Cherie R. Rebar,Nicole M. Heimgartner

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Medical-Surgical Nursing - E-Book

By: Donna D. Ignatavicius,M. Linda Workman,Cherie R. Rebar,Nicole M. Heimgartner

Master the essential medical-surgical nursing content you'll need for success on the Next Generation NCLEX® Exam (NGN) and safe clinical practice! Medical-Surgical Nursing: Concepts for Interprofessional Collaborative Care, 10th Edition uses a conceptual approach to provide adult health knowledge and help you develop the clinical nursing judgment skills that today's medical-surgical nurses need to deliver safe, effective care. "Iggy" emphasizes three emerging trends in nursing — interprofessional collaborative care, concept-based learning, and clinical judgment and systems thinking — trends that will ground you in how to think like a nurse and how to apply your knowledge in the classroom, simulation laboratory, and clinical settings. A perennial bestseller, "Iggy" also features NCLEX Exam-style Challenge and Mastery questions to prepare you for success on the NGN! - Consistent use of interprofessional terminology promotes interprofessional collaboration through the use of a common healthcare language, instead of using isolated nursing-specific diagnostic language. - UNIQUE! Enhanced conceptual approach to learning integrates nursing concepts and exemplars, providing a foundation in professional nursing concepts and health and illness concepts, and showing their application in each chapter. - Unparalleled emphasis on clinical reasoning and clinical judgment helps you develop these vital skills when applying concepts to clinical situations. - Emphasis on QSEN and patient safety focuses on safety and evidence-based practice with Nursing Safety Priority boxes, including Drug Alert, Critical Rescue, and Action Alert boxes. - Direct, easy-to-read writing style features concise sentences and straightforward vocabulary. - Emphasis on health promotion and community-based care reflects the reality that most adult health care takes place in environments outside of high-acuity (hospital) settings.

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1635

Publisher

Elsevier Health Sciences

Published Date

ISBN 10

0323654045

ISBN 13

9780323654043

Forgotten Children

Parent-Child Relations from 1500 to 1900

By: Linda A. Pollock

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Forgotten Children

Parent-Child Relations from 1500 to 1900

By: Linda A. Pollock

The history of childhood is an area so full of errors, distortion and misinterpretation that I thought it vital, if progress were to be made, to supply a clear review of the information on childhood contained in such sources as diaries and autobiographies.' Dr Pollock's statement in her Preface will startle readers who have not questioned the validity of recent theories on the evolution of childhood and the treatment of children, theories which see a movement from a situation where the concept of childhood was almost absent, and children were cruelly treated, to our present western recognition that children are different and should be treated with love and affection. Linda examines this thesis particularly through the close and careful analysis of some hundreds of English and American primary sources. Through these sources, she has been able to reconstruct, probably for the first time, a genuine picture of childhood in the past, and it is a much more humane and optimistic picture than the current stereotype. Her book contains a mass of novel and original material on child-rearing practices and the relations of parents and children, and sets this in the wider framework of developmental psychology, socio-biology and social anthropology. Forgotten Children admirably fulfils the aim of its author. In the face of this scholarly and elegant account of the continuity of parental care, few will now be able to argue for dramatic transformations in the twentieth century.

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354

Publisher

Cambridge University Press

Published Date

ISBN 10

0521271339

ISBN 13

9780521271332

Child Psychology and Psychiatry

Frameworks for Practice

By: David Skuse,Helen Bruce,Linda Dowdney,David Mrazek

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Child Psychology and Psychiatry

Frameworks for Practice

By: David Skuse,Helen Bruce,Linda Dowdney,David Mrazek

Written by leading clinicians and research experts in the fields of child development and psychopathology, this book is an authoritative and up to date guide for psychologists, psychiatrists, paediatricians and other professionals working with vulnerable children. The opening chapters outline neurobiological, genetic, familial and cultural influences upon child development, especially those fostering children's resilience and emotional wellbeing. Discussion of the acquisition of social and emotional developmental competencies leads on to reviews of child psychopathology, clinical diagnoses, assessment and intervention. Developed with busy professionals and trainees in mind, it is comprehensively yet concisely written, using visual aids to help the reader absorb information rapidly and easily. This book is an essential purchase for those working or training in all clinical and community child settings.

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392

Publisher

John Wiley & Sons

Published Date

ISBN 10

1119995744

ISBN 13

9781119995746

Evidence-Based Practice: An Integrative Approach to Research, Administration, and Practice

By: Heather R. Hall,Linda A. Roussel

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Evidence-Based Practice: An Integrative Approach to Research, Administration, and Practice

By: Heather R. Hall,Linda A. Roussel

Evidence-Based Practice: An Integrative Approach to Research, Administration, and Practice, Third Edition focuses on how research-based evidence drives scholarly practice.

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461

Publisher

Jones & Bartlett Learning

Published Date

ISBN 10

1284206513

ISBN 13

9781284206517

Institutions of Higher Education

An International Bibliography

By: Linda Sparks

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Institutions of Higher Education

An International Bibliography

By: Linda Sparks

This bibliography brings together in one comprehensive volume citations of books, dissertations, theses, and ERIC microfiche relating to the history of specific institutions of higher education worldwide. All types of postsecondary institutions--two years colleges, liberal arts colleges, seminaries, specialized institutions, and universities--are included. Entries include the following elements when available: author/editor, title, place of publication, publisher, publication date, and number of pages. Citations from 85 countries are included. Entries are by country, dependency, and territory. The United States has been further divided by state. Names of institutions are in English. References are in the language in which they were written. The majority of the citations should be available in a library somewhere in the United States. Obscure sources that may be difficult to obtain have been included because they are often the only citation. All editions of a title as well as older works are included because of their potential value to a researcher. The book should be a part of all college, university, and large public library collections. College of Education faculty members specializing in higher or comparative education will find much of value here.

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Page Count

489

Publisher

Bloomsbury Publishing USA

Published Date

ISBN 10

0313387788

ISBN 13

9780313387784

Phenomenology for Therapists

Researching the Lived World

By: Linda Finlay

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Phenomenology for Therapists

Researching the Lived World

By: Linda Finlay

This book provides an accessible comprehensive exploration of phenomenological theory and research methods and is geared specifically to the needs of therapists and other health care professionals. An accessible exploration of an increasingly popular qualitative research methodology Explains phenomenological concepts and how they are applied to different stages of the research process and to topics relevant to therapy practice Provides practical examples throughout

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325

Publisher

John Wiley & Sons

Published Date

ISBN 10

0470666455

ISBN 13

9780470666456

Medical-Surgical Nursing

Focus on Clinical Judgment

By: Linda Honan

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Medical-Surgical Nursing

Focus on Clinical Judgment

By: Linda Honan

Confidently meet the demands of transitioning students into practice-ready nurses with Medical-Surgical Nursing: Focus on Clinical Judgment, 3rd Edition. Expertly curated by experienced clinician and nursing educator Dr. Linda Honan, this practical approach distills complex concepts down to need-to-know details through the perspective of practicing nurses, establishing a comprehensive foundation in medical-surgical nursing by way of the most commonly encountered conditions and situations. Extensive updates throughout this 3rd Edition broaden your students’ perspectives, cultivate their clinical judgment, and prepare them for success from the Next Generation NCLEX® to the day-to-day challenges of today’s medical-surgical nursing practice.

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4076

Publisher

Lippincott Williams & Wilkins

Published Date

ISBN 10

1975190963

ISBN 13

9781975190965

The Complete Guide to Acquisitions Management

By: Frances C. Wilkinson,Linda K. Lewis,Rebecca L. Lubas

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The Complete Guide to Acquisitions Management

By: Frances C. Wilkinson,Linda K. Lewis,Rebecca L. Lubas

Updated and enhanced, the second edition of this text provides both library students and practitioners with a thorough understanding of procedural and philosophical approaches in acquisitions management. Since the last edition of this text was published over a decade ago, library acquisitions practices have changed dramatically in response to economic and industry changes and the introduction and maturation of new technologies. An essential tool kit, this updated edition covers every aspect of current acquisitions management from organization of acquisitions departments to professional ethics. The step-by-step guide takes you through acquisitions department activities from the beginning of the ordering process through making materials available to the public. You'll learn about the latest acquisitions systems, negotiating bids and RFPs, gifts and exchange, and decisions of permanence. In addition, the book provides expert guidance on relationships with vendors as well as on the publishing industry in general. Key among the updates to this second edition is information about major changes that have occurred recently, including what's new in integrated library systems, electronic resource management, and patron- and demand-driven acquisitions. An appendix offers a wealth of resources on topics related to acquisitions and includes a comprehensive glossary.

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225

Publisher

Bloomsbury Publishing USA

Published Date

ISBN 10

1610697146

ISBN 13

9781610697149

Sustainable Economics for the Anthropocene

The Economic Scale of Global Boundaries (ESGB) Model

By: Leanne Guarnieri,Linda Lee-Davies

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Sustainable Economics for the Anthropocene

The Economic Scale of Global Boundaries (ESGB) Model

By: Leanne Guarnieri,Linda Lee-Davies

This book examines both the need for sustainable economics and the financial practices that will underpin it. The link between rising inequality and the threat to social sustainability is highlighted to create the Economic Scale of Global Boundaries model, which realigns GDP to include quantifiable environmental and social economic gains and losses. The model is applied at both the national and company level to show its practical application for policy and everyday business practice. The impacts of inequality, declining economic growth and the impending deadlines of the Sustainable Development Goals are also discussed in detail. This book aims to highlight how principles of the circular economy and ESG can be utilized to help meet net zero targets. It will be relevant to students, researchers, organizations, and policymakers interested in environmental economics and sustainability and is written to provoke predictive thinking on the global changes ahead.

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255

Publisher

Springer Nature

Published Date

ISBN 10

303131879X

ISBN 13

9783031318795

Pharmacology for Canadian Health Care Practice

By: Kara Sealock,Linda Lane Lilley,Shelly Rainforth Collins,Julie S. Snyder

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Pharmacology for Canadian Health Care Practice

By: Kara Sealock,Linda Lane Lilley,Shelly Rainforth Collins,Julie S. Snyder

Let this outstanding, reader-friendly pharmacology text help guide you through the detailed world of nursing pharmacology. Now in its third edition, Pharmacology for Canadian Health Care Practice covers all the key pharmacology content needed by today's nursing students. Known for its appealing layout, wealth of photos, and helpful boxed features, this engaging text brings important pharmacology concepts to life. The text's popular key drug approach focuses only on the drug information you need to know. Along with its exam preparation and insightful learning strategies, this is your complete pharmacology text!

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1207

Publisher

Elsevier Health Sciences

Published Date

ISBN 10

1771720662

ISBN 13

9781771720663

The Technology Pork Barrel

By: Linda R. Cohen,Roger G. Noll

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The Technology Pork Barrel

By: Linda R. Cohen,Roger G. Noll

American public policy has had a long history of technological optimism. The success of the United States in research and development contributes to this optimism and leads many to assume that there is a technological fix for significant national problems. Since World War II the federal government has been the major supporter of commercial research and development efforts in a wide variety of industries. But how successful are these projects? And equally important, how do economic and policy factors influence performance and are these influences predictable and controllable? Linda Cohen, Roger Noll, and three other economists address these questions while focusing on the importance of R&D to the national economy. They examine the codependency between technological progress and economic growth and explain such matters as why the private sector often fails to fund commercially applicable research adequately and why the government should focus support on some industries and not others. They also analyze political incentives facing officials who enact and implement programs and the subsequent forces affecting decisions to continue, terminate, or redirect them. The central part of this book presents detailed case histories of six programs: the supersonic transport, communications satellites, the space shuttle, the breeder reactor, photovoltaics, and synthetic fuels. The authors conclude with recommendations for program restructuring to minimize the conflict between economic objectives and political constraints.

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428

Publisher

Brookings Institution Press

Published Date

ISBN 10

0815723687

ISBN 13

9780815723684

Communication and Aging: Creative Approaches to Improving the Quality of Life

By: Linda S. Carozza

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Communication and Aging: Creative Approaches to Improving the Quality of Life

By: Linda S. Carozza

Communication and Aging: Creative Approaches to Improving the Quality of Life provides an overview of alternative approaches used to improve the quality of life of individuals with long-term chronic communication diseases associated with aging and neurogenic diseases. This text examines how professionals can inspire and develop programs that allow patients to live successfully with their disorders. -- from back cover.

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337

Publisher

Plural Publishing

Published Date

ISBN 10

1597568856

ISBN 13

9781597568852

The Baculovirus Expression System

A laboratory guide

By: Linda King

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The Baculovirus Expression System

A laboratory guide

By: Linda King

The decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora tories requiring assistance to find the elusive polyhedrin-negative virus containing their favourite gene. We have also organized two expression system workshops and from the manuals we wrote for these, it seemed a logical progression to extend them into book form. We appreciate that those who are 'old-hands' at the baculovirus expression system may have differing views on some of our procedures, but the methods in this book are presented in the light of our own experiences in the laboratory and from our practical workshops, and we hope that the book will be especially useful to those new to the system. The first three chapters give the background information to the baculovirus expression system, and includes advice on how to choose the right transfer vector and discusses the various methods that are available to select recombinant viruses. The practical chapters concentrate on those aspects which are novel to the baculovirus system (insect cell culture, virus amplification and titration, etc. ) and, in general, leave the standard molecular biological techniques to the other excellent laboratory manuals that are available. However, for completeness sake and to avoid constant reference to other manuals, we have included brief details of some standard techniques where they are integral to the success of the baculovirus protocols.

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242

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Springer Science & Business Media

Published Date

ISBN 10

9401123748

ISBN 13

9789401123747

Book's by Linda C. Smith

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