This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.
In When Prophets Preach: Leadership and the Politics of the Pulpit, Jay Augustine urges twenty-first-century preachers to speak openly against social injustice, establishing such preaching as a key component of prophetic leadership. Beginning with the premise that the church was birthed to address salvation in the "kingdom-to-come" and social justice in the "kingdom-at-hand," Augustine presents prophetic preaching as part of the ministry of reconciliation Jesus left to the church. Addressing topics such as abusive immigration policies and racial injustices, he urges the church to return to its foundation of prophetic leadership as exemplified not only by Jesus but by the Old Testament prophets and the New Testament apostles. When Prophets Preach demonstrates that faithfulness to this ministry requires preachers to break the pulpit silence. Then the church can lead in bridging social and ethnic gaps among its members. It can show society at large how to heal many of the social, economic, and political divisions in our world, the very rifts that underscore the need for social justice ministries and that necessitate prophetic preaching.
First published in 2009. From the founding of Jamestown to the American Civil War, slavery and abolition shaped American national, regional and racial identities. This four-volume reset edition draws together rare sources relating to American slavery systems. Volume 3 includes the Antebellum Period from 1828 to 1859.
Now in its second edition, this practical guide offers clear-headed guidance to the successful application of catheter ablation for atrial fibrillation. This book concentrates on clinically-relevant information that providers can put to immediate use caring for patients. Takes a clear-headed practical approach to ablation of atrial fibrillation – long on actionable, clinically-relevant guidance, succinct and to-the-point on the theory behind the procedure Edited by three leading, internationally-known electrophysiologists with extensive experience in ablation for atrial fibrillation Written by international team of experts reflecting global best practices from centers with considerable experience in the use of catheter ablation Format designed to serve the needs of electrophysiologists regardless of experience, electrophysiology fellows, electrophysiology nurses and lab technical staff Covers hot topics such as new noninvasive imaging techniques, the treatment of challenging left atrial flutters, options for persistent atrial fibrillation and when a redo ablation is needed; and novel application of ablation targeting the autonomic nervous system
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