The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
1 John H. Dodds The culture offragmen ts of plant tissue is not a particularly new science, in fact as long ago as 1893 Rechinger (1893) described the formation of callus on isolated fragments of stems and roots. The culture of plant tissues in vitro on a nutrient medium was performed by Haberlandt (1902), however, his attempts were unsuccessful because he chose too simple a medium that lacked critical growth factors. Over the last fifty years there has been a surge of development in plant tissue culture techniques and a host of techniques are now avail able (Dodds and Roberts, 1982). The major areas are as follows. Callus Culture Callus is a rather ill-dermed material. but is usually described as an un organised proliferating mass of tissue. Although callus cultures have a great deal of potential in the biotechnological aspects of tissue culture, i.e. secondary product formation, they are not very suitable for plant propagation. The key reason for their unsuitability is that genetic aber rations occur during mitotic divisions in callus growth (D'amato.l965). The aberrations can be of a major type, such as aneuploidy or endo reduplication. It follows therefore that the genetic status of the re generated plants is different from that of the parent type. In general terms this genetic instability is undesirable, but there are occasions when a callus stage can be purposely included to diversify the genetic base of the crop.
Outskirts of Empire: Studies in British Power Projection investigates the substructure of Britain’s interests in the Near East and beyond during the nineteenth and twentieth centuries. Essays address themes in British power projection in a geographically wide area encompassing parts of the Ottoman Empire, Morocco and Abyssinia, illuminating interlinking elements of Britain’s power and presence through commerce, religion, consular activity, expatriates, travel and exploration and technology. Through careful investigation of the interface of these themes the book develops a deeper sense of Britain’s presence in the Near East and contiguous areas and highlights the network of Britons who were required to sustain that presence.
The Georgia 4th Cavalry Regiment (AveryÕs) was formed with eleven companies in January, 1863, using Avery's 23rd Georgia Cavalry Battalion as its nucleus. It served for a time with the Conscript Department in Tennessee, then was assigned to J.J. Morrison's, C.C. Crews', and Iverson's Brigade. The regiment participated in the Chickamauga, Knoxville, and Atlanta Campaigns, skirmished in Northern Georgia and East Tennessee, and took part in the defense of Savannah. In January, 1865, the unit was reorganized and called the 12th Cavalry. It went on to fight in the Carolinas and surrendered with the Army of Tennessee. Research of this unit is complicated by the fact that there was another 4th Cavalry (ClinchÕs). No roster of the 23rd Cavalry Battalion or the 4th Cavalry (AveryÕs) has been found. The records are all filed in the state archive microfilm as the GA 12th Cavalry Regiment.
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