Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. - Offers project lab formats for students that closely simulate original research projects - Provides instructional guidance for students to design their own experiments - Includes advanced analytical techniques - Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH - Includes access to a website with additional resources for instructors
Describes proteins' physical and chemical nature and how their molecular structures can be determined experimentally. Intended for upper level undergraduate and graduate students with a background in chemistry or biochemistry.
The second edition of Structure in Protein Chemistry showcases the latest developments and innovations in the field of protein structure analysis and prediction. The book begins by explaining how proteins are purified and describes methods for elucidating their sequences of amino acids and defining their posttranslational modifications. Comprehensive explanations of crystallography and of noncovalent forces-ionic interactions, hydrogen bonding, and the hydrophobic effect-act as a prelude to an exhaustive description of the atomic details of the structures of proteins. The resulting understanding of protein molecular structure forms the basis for discussions of the evolution of proteins, the symmetry of the oligomeric associations that produce them, and the chemical, mathematical, and physical basis of the techniques used to study their structures. The latter include image reconstruction, nuclear magnetic resonance spectroscopy, proton exchange, optical spectroscopy, electrophoresis, covalent cross-linking, chemical modification, immunochemistry, hydrodynamics, and the scattering of light, X-radiation, and neutrons. These procedures are applied to study the folding of polypeptides and the assembly of oligomers. Biological membranes and their proteins are also discussed. Structure in Protein Chemistry, Second Edition, bridges the gap between introductory biophysical chemistry courses and research literature. It serves as a comprehensive textbook for advanced undergraduates and graduate students in biochemistry, biophysics, and structural and molecular biology. Professionals engaged in chemical, biochemical, and molecular biological research will find it a useful reference.
Jack Kerouac’s classic novel about friendship, the search for meaning, and the allure of nature “In [On the Road] Kerouac’s heroes were sensation seekers; now they are seekers after truth . . . the novel often attains a beautiful dignity.”—Chicago Tribune First published in 1958, a year after On the Road put the Beat Generation on the map, The Dharma Bums stands as one of Jack Kerouac’s most powerful and influential novels. The story focuses on two ebullient young Americans—mountaineer, poet, and Zen Buddhist Japhy Ryder, and Ray Smith, a zestful, innocent writer—whose quest for Truth leads them on a heroic odyssey, from marathon parties and poetry jam sessions in San Francisco’s Bohemia to solitude and mountain climbing in the High Sierras.
Jack Kerouac was an American novelist, poet and leader of the Beat movement. His iconic masterpiece ‘On the Road’ exacted a broad cultural influence, capturing the spirit of its time as no other work of the 20th century had done since ‘The Great Gatsby’. Kerouac’s insistence upon ‘First thought, best thought’ and his refusal to revise was controversial. He deemed revision as a form of literary lying, imposing a form farther away from the truth of the moment. His novels reveal a quest for pure, unadulterated language—the truth of the heart unobstructed by the lying of revision. His technique demonstrates an unusual writing style, neither haphazard nor sloppy, but systematic in the most-individualised sense. For the first time in publishing history, this eBook presents Kerouac’s complete fictional works, with numerous illustrations, rare texts appearing in digital print for the first time, informative introductions and bonus material. (Version 1)* Beautifully illustrated with images relating to Kerouac’s life and works * Concise introductions to the major texts * All 15 novels and novellas, with individual contents tables * Features rare novels appearing for the first time in digital publishing * Images of how the books were first published, giving your eReader a taste of the original texts * Excellent formatting of the texts * Rare poetry texts * Easily locate the poems you want to read * Includes Kerouac’s seminal non-fiction collection, ‘Lonesome Traveler’ * Ordering of texts into chronological order and genresPlease note: the poetry published after Kerouac’s death cannot appear in this edition, due to copyright restrictions.CONTENTS:The Novels The Town and the City (1950) On the Road (1957) The Dharma Bums (1958) Doctor Sax (1959) Maggie Cassidy (1959) Book of Dreams (1960) Big Sur (1962) Visions of Gerard (1963) Desolation Angels (1965) Vanity of Duluoz (1968) Visions of Cody (1972)The Novellas The Subterraneans (1958) Tristessa (1960) Satori in Paris (1966) Pic (1971)The Poetry Mexico City Blues (1959) The Scripture of the Golden Eternity (1960) Old Angel Midnight (1973)The Non-Fiction Lonesome Traveler (1960)Please visit www.delphiclassics.com to browse through our range of exciting titles or to purchase this eBook as a Parts Edition of individual eBooks
The Boys' Adventure Megapack collects 20 great adventure stories for boys, from classics like "The Call of the Wild" to war stories like the adventures of Dave Dawson, from science fiction like Tom Swift, Jr. to historical fiction like Tarzan of the Apes and The Count of Monte Cristo. Rollicking adventure stories for the young and young at heart! Included are: THE CALL OF THE WILD, by Jack London TREASURE ISLAND, by Robert Louis Stevenson KIDNAPPED, by Robert Louis Stevenson THE SWISS FAMILY ROBINSON, by Johann David Wyss FIVE CHILDREN AND IT, by E. Nesbit TOM SWIFT AND THE VISITOR FROM PLANET X, by Victor Appleton II TOM SWIFT AND THE ELECTRONIC HYDROLUNG, by Victor Appleton II TARZAN OF THE APES, by Edgar Rice Burroughs THE RETURN OF TARZAN, by Edgar Rice Burroughs DAVE DAWSON AT DUNKIRK, by R. Sidney Bowen DAVE DAWSON WITH THE R.A.F., by R. Sidney Bowen DAVE DAWSON ON THE RUSSIAN FRONT, by R. Sidney Bowen DAVE DAWSON ON GUADALCANAL, by R. Sidney Bowen DAVE DAWSON AT CASABLANCA, by R. Sidney Bowen DAVE DAWSON AT TRUK, by R. Sidney Bowen DAVE DASHAWAY AND HIS HYDROPLANE, by Roy Rockwood ADRIFT IN THE WILDS: THE ADVENTURES OF TWO SHIPWRECKED BOYS, by Edward S. Ellis AMONG MALAY PIRATES, by G. A. Henty KIM, by Rudyard Kipling THE COUNT OF MONTE CRISTO, by Alexandre Dumas And don't forget to search your favorite ebook store for more entries in Wildside Press's Megapack series, ranging from science fiction and fantasy to westerns, mysteries, ghost stories -- and much, much more!
Not just another science book and not just another Discworld novella, The Science of Discworld is a creative, mind-bending mash-up of fiction and fact, that offers a wizard’s-eye view of our world that will forever change how you look at the universe. Can Unseen University’s eccentric wizards and orangutan Librarian possibly shed any useful light on hard, rational Earthly science? In the course of an exciting experiment, the wizards of Discworld have accidentally created a new universe. Within this universe is a planet that they name Roundworld. Roundworld is, of course, Earth, and the universe is our own. As the wizards watch their creation grow, Terry Pratchett and acclaimed science writers Ian Stewart and Jack Cohen use Discworld to examine science from the outside. Interwoven with the Pratchett’s original story are entertaining, enlightening chapters which explain key scientific principles such as the Big Bang theory and the evolution of life on earth, as well as great moments in the history of science.
One of the classic works of marine biology, a favorite for generations, has now been completely revised and expanded. Between Pacific Tides is a book for all who find the shore a place of excitement, wonder, and beauty, and an unsurpassed introductory text for both students and professionals. This book describes the habits and habitats of the animals that live in one of the most prolific life zones of the world--the rocky shores and tide pools of the Pacific Coast of the United States. The intricate and fascinating life processes of these creatures are described with affectionate care. The animals are grouped according to their most characteristic habitat, whether rocky shore, sandy beach, mud flat, or wharf piling, and the authors discuss their life history, physiology, and community relations, and the influence of wave shock and shifting tide level. Though the basic purpose and structure--and much of the text--of the book remain the same, content has been increased by about 20 percent; a multitude of changes and additios has been made in the text; the Annotated Systematic Index and General Bibliography have been updated and greatly expanded (now almost 2,300 entries); more than 200 new photographs and drawings have been incorporated; and an entirely new chapter has been added--a topical presentation of the several factors influencing distribution of organisms along the shore. This edition also includes John Steinbeck's Foreword to the 1948 edition.
Popular amongst students and practitioners, Anson's Law of Contract is a well-established and well-respected classic of contract law.Written by three of the foremost experts in the field, it provides an authoritative account of the subject. Detailed, yet clear, the book leads readers through extensive explanations and analyses of the key underlying principles of contract law.Thoroughly updated to incorporate the most recent legislation and case law, this definitive work is essential reading on contract law.
The second edition of Structure in Protein Chemistry showcases the latest developments and innovations in the field of protein structure analysis and prediction. The book begins by explaining how proteins are purified and describes methods for elucidating their sequences of amino acids and defining their posttranslational modifications. Comprehensive explanations of crystallography and of noncovalent forces-ionic interactions, hydrogen bonding, and the hydrophobic effect-act as a prelude to an exhaustive description of the atomic details of the structures of proteins. The resulting understanding of protein molecular structure forms the basis for discussions of the evolution of proteins, the symmetry of the oligomeric associations that produce them, and the chemical, mathematical, and physical basis of the techniques used to study their structures. The latter include image reconstruction, nuclear magnetic resonance spectroscopy, proton exchange, optical spectroscopy, electrophoresis, covalent cross-linking, chemical modification, immunochemistry, hydrodynamics, and the scattering of light, X-radiation, and neutrons. These procedures are applied to study the folding of polypeptides and the assembly of oligomers. Biological membranes and their proteins are also discussed. Structure in Protein Chemistry, Second Edition, bridges the gap between introductory biophysical chemistry courses and research literature. It serves as a comprehensive textbook for advanced undergraduates and graduate students in biochemistry, biophysics, and structural and molecular biology. Professionals engaged in chemical, biochemical, and molecular biological research will find it a useful reference.
Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. - Offers project lab formats for students that closely simulate original research projects - Provides instructional guidance for students to design their own experiments - Includes advanced analytical techniques - Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH - Includes access to a website with additional resources for instructors
Vine royalty, YouTube megastars, hip-pop sensations, and best friends, Jack & Jack bring their own brand of irreverent comedy, on-point style, and heartfelt life advice to You Don't Know Jacks. Jack & Jack: You Don't Know Jacks is a 240-page, full-colour behind-the-scenes look at the lives of Jack Gilinsky and Jack Johnson, two of the hottest stars performing today. The book details the rise of two best friends growing up in Nebraska, posting Nerd Vandals Vines, to becoming iTunes bestselling rap-rock stars. Full of exclusive photographs, backstage antics, and hilarious anecdotes, it's perfect for any fan who's ever dreamed of someday being famous.
These tales have appeared in "McClure's," "Ainslee's," "Outing," the "Overland Monthly," the "Wave," the "National," and the San Francisco "Examiner." To the kindness of the various editors is due their reappearance in more permanent form.To the Daughters of the Wolf Who Have Bred and Suckled a Race of MenThe God of His FathersThe Great InterrogationWhich Make Men RememberSiwashThe Man with the GashJan, the UnrepentantGrit of WomenWhere the Trail ForksA Daughter of the AuroraAt the Rainbow's EndThe Scorn of Women
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