This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture
Introduces students to the key concepts and challenges in this topical area by exploring and challenging the notion of sustainability and its relationship to contemporary tourism in the developing world.
Ian Hodder’s campaigns of excavation at the world-famous Neolithic settlement of Çatalhöyük are one of the largest, most complex, and most exciting archaeological field projects in the world and recognized as agenda-setting not only in terms of our understanding of early farming communities in the Near East, particularly the central role religion played in their daily lives, but also in terms of the interaction between theory and practice in the trenches and on-site laboratories. This volume presents the results of excavation in three areas of the site, known as South, North, and KOPAL, excavated between 1995 and 1999. The book describes aspects of the excavation, recording and sampling methodologies that are necessary for an understanding of the results presented plus it incorporates interpretive discussion. It brings in data from the study of animal bones, lithics, ceramics, micromorphology and the full suite of analyses conducted on the material. These accounts are interspersed with individual specialists’ commentaries and conclusions, that mimic the process of collaborative interpretation that takes place during excavation and post-excavation. The ‘objective descriptions’ of the archaeology are thus exposed as interpretations involving a balancing of a variety of different types of data and scholarly input. Another thought-provoking volume in the Çatalhöyük excavation series which will be read with profit by any archaeologist engaged in working at theory in practice in the field.
Combining breadth of coverage with detail, this logical and cohesive introduction to insect ecology couples concepts with a broad range of examples and practical applications. It explores cutting-edge topics in the field, drawing on and highlighting the links between theory and the latest empirical studies. The sections are structured around a series of key topics, including behavioral ecology; species interactions; population ecology; food webs, communities and ecosystems; and broad patterns in nature. Chapters progress logically from the small scale to the large; from individual species through to species interactions, populations and communities. Application sections at the end of each chapter outline the practicality of ecological concepts and show how ecological information and concepts can be useful in agriculture, horticulture and forestry. Each chapter ends with a summary, providing a brief recap, followed by a set of questions and discussion topics designed to encourage independent and creative thinking.
Archaelogists and anthropologists (especially ethnologists) have for many years realised that man's ingestion of alcoholic beverages may well have played a significant part in his transition from hunter-gatherer to agriculturalist. This unique book provides a scientific text on the subject of 'ethanol' that also aims to include material designed to show 'non-scientists' what fermentation is all about. Conversely, scientists may well be surprised to find the extent to which ethanol has played a part in evolution and civilisation of our species.
Stemming from an 11-year DFID funded programme under its Renewable Natural Resources Research Strategy (RNRRS), Technology Development Assistance for Agriculture: Putting Research into Low Income Countries reviews part of this programme as a case study of a broader issue of technology development for Africa. Controversially, it critiques current international technology development assistance and focuses on the potential role of the private sector in agricultural technology development as well as providing insights for future cognate science policy and practice. The book focuses on the RIU "Best Bets" Africa sub-programme. This identified promising proposals to take existing agriculture research products and put these into use in ways that would benefit the poor in developing countries. The sum set aside for this was £5 million. The empirical sections of the book cover project selection, progress and programme management over a 2009-2012 period with special attention paid to lessons learned that may have implications for future cognate technology development assistance. This topical book gives direct evidence of meeting objectives and delivering real changes in technology development for Africa to postgraduate students, researchers, international bodies, NGOs, policy makers and government organisations working on natural resource management, technology development assistance, and low income country agriculture.
An overview of the way the archaeological debate has developed over the last 10 years. Hodder aims to break down the separation between theory and practice and reconcile the division between the intellectual and the 'dirt' archaeologist.
Advanced Therapy of Prostate Disease, from the inital to post-surgical psychological concerns, this book is a complete guide to every step of prostate disease treatment. First, it describes the physical exam in detail, as well as laboratory and imaging techniques that can confirm a diagnosis. Then, the pros and cons of treatment methods for every type and variation of prostate cancer and benign conditions are discussed. Post-surgical treatment (including behavioral issues) is also outlined.
Cultivate an interest in the agricultural sector with a three-level secondary course designed specifically for the Caribbean. - Explore regional and global practices and developments in agriculture. - Review career options in an increasingly lucrative and essential sector. - Enhance understanding of the relevance of agriculture with a project-based approach to select topics. - Prepare for study at the CSEC level with a dedicated project-based chapter scalable to other topics and SBA research at the CSEC level. - Consolidate learning with a clear chapter objectives and end of chapter evaluation.
Reap the advantages of backyard-to-table produce with tips on planning, soil, fertilizers, cultivation, pests, and diseases. Includes a Quickstart Guide! This is a simple and systematic guide to growing a selection of the tastiest fruit and vegetables. The aim of this book is to start you off with some easy-to-grow produce such as carrots, onions, radishes, tomatoes and strawberries. Once you have the confidence of the first growing season behind you, you can then progress to crops requiring slightly more labor, such as peas, beans and raspberries. When you grow your own produce, you can be absolutely sure that everything has been organically cared for and you can grow just the variety you like. You can pick the fruit and vegetables at their freshest without a tiresome journey to the supermarket. It’s satisfying, it’s economical and it’s delicious.
This much-awaited final volume of The Birds of British Columbia completes what some have called one of the most important regional ornithological works in North America. It is the culmination of more than 25 years of effort by the authors who, with the assistance of thousands of dedicated volunteers throughout the province, have created the basic reference work on the avifauna of British Columbia.
This book meets the demand for a comprehensive introduction to understanding the processes of population limitation. Recognized world-wide as a respected biologist and communicator, Dr. Ian Newton has now written a clear and detailed treatise on local scale population limiting factors in birds. It is based almost entirely on results from field studies, though it is set in a contemporary theoretical framework. The 16 chapters fall under three major section headings: Behavior and Density Regulation; Natural Limiting Factors; and Human Impacts. Population Limitation in Birds serves as a needed resource expanding on Dr. David Lacks research in this area of ornithology in the 1950s. It includes numerous line diagrams and beautiful illustrations by acclaimed wildlife artist Keith Brockie. - Provides a sorely needed introduction to a long-established core subject in ornithology - Focuses on local scale factors - Written by a well-known biologist and effective communicator - Includes numerous line diagrams and beautiful illustrations by acclaimed wildlife artist Keith Brockie
New or recently sterilized islands (for example through volcanic activity), provide ecologists with natural experiments in which to study colonization, development and establishment of new biological communities. Studies carried out on islands like this have provided answers to fundamental questions as to what general principles are involved in the ecology of communities and what processes underlie and maintain the basic structure of ecosystems. These studies are vital for conservation biology, especially when evolutionary processes need to be maintained in systems in order to maintain biodiversity. The major themes are how animal and plant communities establish, particularly on 'new land' or following extirpations by volcanic activity. This book comprises a broad review of island colonization, bringing together succession models and general principles, case studies with which Professor Ian Thornton was intimately involved, and a synthesis of ideas, concluding with a look to the future for similar studies.
So long as you have food in your mouth, you have solved all questions for the time being. So begins Good Enough to Eat?, which challenges Kafka’s culinary sentiments and proceeds to unravel our complex and deeply personal relationship with food. Including interviews from both sides of the (farmyard) fence; from biologists to farmers and nutritionists to activists, Good Enough to Eat? charts the history of GM foods from the laboratory to the global dinner plate. Equally informative and entertaining, Godwin chronicles the social, political and philosophical arguments for and against GM crops, and the science and knowledge behind the battle for global food security and sustainability.
After the excitement of its discovery and excavations in the early 1960s, the world-important site of Çatalhöyük has remained dormant for 30 years. This is Volume 1 of the Çatalhöyük Research Project series. It describes the first phase of renewed archaeological research at the site. It reports on the work that has taken place on the surfaces of the east and west mounds and in the surrounding regions. It also discusses the material from the 1960s excavation in museums, which has been re-examined. The result is that new perspectives can be offered on the internal organization and symbolism of a site which is central to our understanding of the earliest development of complex societies.
Deeply insightful, sensitive and passionate. An inspiring, meticulous picture of the innovations that have made us the world's oldest living culture.' - Larissa Behrendt 'Another fascinating volume in this landmark Australian publishing series.' - Richard Flanagan What do you need to know to prosper as a people for at least 65,000 years? The First Knowledges series provides a deeper understanding of the expertise and ingenuity of Indigenous Australians. First Nations Australians are some of the oldest innovators in the world. Original developments in social and religious activities, trading strategies, technology and land-management are underpinned by philosophies that strengthen sustainability of Country and continue to be utilised today. Innovation: Knowledge and Ingenuity reveals novel and creative practices such as: body shaping; cremation; sea hunting with the help of suckerfish; building artificial reefs for oyster farms; repurposing glass from Europeans into spearheads; economic responses to colonisation; and a Voice to Parliament. In the first book to detail Indigenous innovations in Australia, Ian J McNiven and Lynette Russell showcase this legacy of First Nations peoples and how they offer resourceful ways of dealing with contemporary challenges that can benefit us all. *Ebook available through all major etailers*
New Wild Garden shows how to adapt an environmentally conscious new style to your garden, whatever its size and aspect, using easy-to-grasp techniques, planting ideas and schemes.
FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.
Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology. The first section includes experiments on the soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens--bacteria, viruses, and protozoan parasites--used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation. Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material. - Incorporates new experiments and the latest techniques - Designed for maximum versatility and ease of use for the student and instructor - Each experiment is self-contained and includes theoretical, practical, and pedagogical material
Using the experiences of large water infrastructure projects involving the transfer of water from basins considered to have surplus water to those where the demand for water has exceeded or is expected to exceed supplies, this book examines case studies within diverse geographical, climatic, economic, and policy regimes.
The world struggles with increasing threats to global sustainability, caused by population growth, overuse of fresh water resources, depletion of biodiversity, and reliance on non-renewable energy sources. There is an urgent need for an overall plan to address these challenges in a coordinated and effective manner. Whether in government, business, community or as an individual, we need to begin acting a lot smarter, faster and more collaboratively if we are going to avert the potential devastating impacts on this planet. Plan for the Planet outlines a co-ordinated approach to tackling the global challenges we face which can be implemented at every level. Using proven business management wisdom and principles, this book provides perhaps the most comprehensive and robust framework within which business, government and the community can work together to build a sustainable world. Whether you want to understand how to prepare your organisation and yourself to deal successfully with the global challenges, or seize the opportunities which are fast developing with the emergence of the sustainability revolution, you will benefit from reading this timely book.
The world struggles with increasing threats to global sustainability, caused by population growth, overuse of fresh water resources, depletion of biodiversity, and reliance on non-renewable energy sources. This title outlines a co-ordinated approach to tackling the global challenges we face which can be implemented at every level.
Assess your readiness for MCTS Exam 70-662—and quickly identify where you need to focus and practice. This practical, streamlined guide walks you through each exam objective, providing "need to know" checklists, review questions, tips, and links to further study—all designed to help bolster your preparation. Reinforce your exam prep with a Rapid Review of these objectives: Installing and Configuring Exchange Servers Configuring Exchange Recipients and Public Folders Configuring Client Access Configuring Message Transport Monitoring and Reporting Implementing High Availability and Recovery Configuring Message Compliance and Security Features This book is an ideal complement to the in-depth training of the Microsoft Press Training Kit and other exam-prep resources for Exam 70-662.
In Six Thousand Years up the Garden Path, a seasoned horticulturalist takes others on a light-hearted and magical journey through the history of gardens. Ian Robertson has spent a lifetime working in the world of plants and design and relies on his experience as an international garden designer to narrate a fascinating history of gardening. Beginning with Sumerians learning to garden between the Tigris and Euphrates rivers, Robertson transports others through the Fertile Crescent to western Europe, forward to the British Isles, and onward to the New World all while describing the beauty of the gardens of Andrew Jackson Downing, Wolfgang Oehme, Frederick Law Olmstead, and many others. Included in his story of the foliage and blossoms that surrounded kings, princes, queens, and mistresses, Robertson shares a timeline, glossary, and roadside stopovers that make the journey up the garden path even more delightful. Tailored for the enjoyment of either experienced or novice gardeners immersed in the second fastest growing hobby in America, Robertson provides insights into the people, events, and plants that have vibrantly colored the landscape of the world for thousands of years.
The world struggles with increasing threats to global sustainability, caused by population growth, overuse of fresh water resources, depletion of biodiversity, and reliance on non-renewable energy sources. There is an urgent need for an overall plan to address these challenges in a coordinated and effective manner. Whether in government, business, community or as an individual, we need to begin acting a lot smarter, faster and more collaboratively if we are going to avert the potential devastating impacts on this planet. Plan for the Planet outlines a co-ordinated approach to tackling the global challenges we face which can be implemented at every level. Using proven business management wisdom and principles, this book provides perhaps the most comprehensive and robust framework within which business, government and the community can work together to build a sustainable world. Whether you want to understand how to prepare your organisation and yourself to deal successfully with the global challenges, or seize the opportunities which are fast developing with the emergence of the sustainability revolution, you will benefit from reading this timely book.
William Miller details our anxious relation to basic life processes; eating, excreting, fornicating, decaying, and dying. But disgust pushes beyond the flesh to vivify the larger social order with the idiom it commandeers from the sights, smells, tastes, feels, and sounds of fleshly physicality. Disgust and contempt, Miller argues, play crucial political roles in creating and maintaining social hierarchy. Democracy depends less on respect for persons than on an equal distribution of contempt. Disgust, however, signals dangerous division.
This updated edition of Professor Ian Hodders original and classic work on the role which anthropology must play in the interpretation of the archaeological record.There has long been a need for archaeologists and anthropologists to correlate their ideas and methods for interpreting the material culture of past civilisations. Archaeological interpretation of the past is inevitably based on the ideas and experiences of the present and the use of such ethnographic analogy has been widely adapted and criticised, not least in Britain.In this challenging study, Ian Hodder questions the assumptions, values and methods which have been too readily accepted. At the same time, he shows how anthropology can be applied to archaeology. He examines the criteria for the proper use of analogy and, in particular, emphasises the need to consider the meaning and interpretation of material cultures within the total social and cultural contexts. He discusses anthropological models of refuse deposits, technology and production, subsistence, settlement, burial, trade exchange, art form and ritual; he then considers their application to comparable archaeological data.Throughout, Professor Hodder emphasises the need for a truly scientific approach and a critical self-awareness by archaeologists, who should be prepared to study their own social and cultural context, not least their own attitudes to the present-day material world.
Linking environmental sustainability with poverty reduction and social justice, and making science and technology work for the poor, have become central practical, political and moral challenges of our times. These must be met in a world of rapid, interconnected change in environments, societies and economies, and globalised, fragmented governance arrangements. Yet despite growing international attention and investment, policy attempts often fail. Why is this, and what can be done about it? How might we understand and address emergent threats from epidemic disease, or the challenges of water scarcity in dryland India? In the context of climate change, how might seed systems help African farmers meet their needs, and how might appropriate energy strategies be developed? This book lays out a new 'pathways approach' to address sustainability challenges such as these in today's dynamic world. Through an appreciation of dynamics, complexity, uncertainty, differing narratives and the values-based aims of sustainability, the pathways approach allows us to see how some approaches are dominant, even though they do not produce the desired results, and how to create successful alternative 'pathways' of responding to the challenges we face. As well as offering new ways of thinking about sustainability, the book also suggests a series of practical ways forward - in tools and methods, forms of political engagement, and styles of knowledge-making and communication. Throughout the book, the practicalities of the pathways approach are illustrated using four case studies: water in dryland India, agricultural seeds in Africa, responses to epidemic disease and energy systems/climate change. Published in association with the Economic and Social Research Council (ESRC)
This is a challenging new approach to understanding ecological systems especially in urban and urbanised areas. Synthesising current ideas and approaches the book develops an historic context to ecological fusion and recombinant or hybrid ecosystems. With massive climate change and other environmental fluxes, this volume provides insight into consequences for future ecologies. Invasive and non-native or alien species are spreading, often aggressively around the globe. However, much current thinking in ecology and nature conservation fails to accommodate the consequences of changing environmental conditions and fusion of both species and ecological communities. Whether or not conservationists accept ecological change, factors such as urbanisation and globalisation combine with climate and other changes to trigger new hybrid communities and ecologies. Embedding this approach into current ecological thinking this book presents an overview of ideas set in the exemplar case study area of the British Isles. However, the approaches, ideas and conclusions presented here will find application in ecosystem studies and in nature conservation around the world.
This textbook presents an overview of how the activities of an organisation can be managed to satisfy the needs of stakeholders through the cost effective, operationally efficient and sustainable transformation of resources into outputs. Taking an interdisciplinary approach, the authors show the relationship between management and economics and within this framework present the key areas of management activity. The book explains the connections between these areas and provides tools and instruments for successful management. The book's approach and content is relevant for all kinds of organisation - private or public sector, service or manufacturing, non-profit, large or small. Each chapter provides cases to illustrate what has been discussed and some questions to test comprehension. Throughout the book is a continuing project in which the reader is put in the position of owning their own business and must think and make decisions about what the chapter has discussed. The book combines Anglo-American and German approaches to management and management studies, making it a valuable resource both for those who are studying management and those who are working as managers.
This book introduces to the English-speaking world the leading modern Islamic thinker Said Nursi (1878-1960) - who has some nine million followers in modern day Turkey and around the world. After an opening chapter that provides an overview of his life, the next four chapters outline the theology of Nursi on God, the Qur'an, the West and Politics. The final section provides an invaluable resource of readings from Nursi's most important writings. Providing an introduction to a major form of Islam which is committed to non-violence, dialogue and constructive relationships with the West, this is the first student textbook to introduce a contemporary Islamic theologian in a systematic way.
Urban Ecology: An Introduction seeks to open the reader’s mind and eyes to the way in which nature permeates everyday urban living, and how it has to be understood, cared for, and managed in order to make our towns and cities healthier places to visit and in which to live and work. The authors examine how nature can improve our physical and mental health, the air we breathe and the waters we use, as well as boosting our enjoyment of parks and gardens. Urban Ecology sets out the science that underlies the changing natural scene and the tools used to ensure that cities become both capable of adapting to climate change and more beautiful and resilient. The book begins with a discussion of the nature of urban places and the role of nature in towns and cities. Part 1 looks at the context and content of urban ecology, its relationship to other foci of interest within ecology and other environmental sciences, and the character of city landscapes and ecosystems. In Part 2 the authors set out the physical and chemical components of urban ecosystems and ecological processes, including urban weather and climate, urban geomorphology and soils, urban hydrology and urban biogeochemical cycles. In Part 3 urban habitats, urban flora and fauna, and the effects of, deliberate and inadvertent human action on urban biota are examined. Part 4 contains an exploration of the identification and assessment of ecosystem services in urban areas, emphasising economic evaluation, the importance of urban nature for human health and well-being, and restoration ecology and creative conservation. Finally, in Part 5 the tasks for urban ecologists in optimising and sustaining urban ecosystems, providing for nature in cities, adapting to climate change and in developing the urban future in a more sustainable manner are set out. Within the 16 chapters of the book – in which examples from around the world are drawn upon - the authors explore current practice and future alternatives, set out procedures for ecological assessment and evaluation, suggest student activities and discussion topics, provide recommended reading and an extensive bibliography. The book contains more than 150 tables and over 150 photographs and diagrams.
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