This practical laboratory manual provides an essential source of reference, information and guidance for all laboratory and clinical immunologists. It fully describes the methods used in diagnostic immunopathology, and discusses the interpretation and value of the parameters measured. It also answers important practical questions: which parameters are useful in arriving at a diagnosis; which are useful for monitoring the severity of a disease; what level of precision is achievable, and what level is useful; how do we measure accuracy, and how do we achieve inter-laboratory consistency? Each chapter has a brief introduction which provides some general comments on the procedures involved. The methods section contains detailed descriptions with helpful notes on the advantages and disadvantages of different methods and potential pitfalls. Finally, each chapter concludes with a section on clinical applications, which discusses the interpretation, value and limitations of the information obtained, and asks what alternative interpretations should be considered, and what additional information is called for.
This book describes, in detail, tested techniques for the produc-tion and use of monoclonal antibodies. It covers those aspects of interest to all scientists working with monoclonal antibodies and presents methods in a step-by-step format for easy refer-ence. The text serves as a laboratory manual; and discusses rationale behind each method, and th
Offers comprehensive coverage of all known CD molecules based on the results of the latest Human Leukocyte Differentiation Antigen (HLDA) Workshops. It begins with an historical introduction, followed by a concise summary of the structure, function, and applications in research, diagnosis and therapy. Next, overviews for each section discuss latest developments, methodologies, and conclusions. The core chapters then offer one-page CD summaries in a standardized format. The summaries present salient features for each molecule, including its structure (accompanied by a diagram), cell and tissue distribution, function, clinical applications, available reagents, and sources for additional information.
Offers comprehensive coverage of all known CD molecules based on the results of the latest Human Leukocyte Differentiation Antigen (HLDA) Workshops. It begins with an historical introduction, followed by a concise summary of the structure, function, and applications in research, diagnosis and therapy. Next, overviews for each section discuss latest developments, methodologies, and conclusions. The core chapters then offer one-page CD summaries in a standardized format. The summaries present salient features for each molecule, including its structure (accompanied by a diagram), cell and tissue distribution, function, clinical applications, available reagents, and sources for additional information.
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