A multiplicity of methods for visualization are described in Visualization of Receptors. This book of techniques covers immunocytology, radioautography, in situ hybridization, plasmon resonance, RT in vitro PCR, and X-ray crystal structure analysis. Lecturers, researchers, practitioners, technicians, and students will find all the principles and protocols they require and the means of implementing them. Topics discussed include localization of ligands (in vitro or in vivo), visualization of immunoreactivities of ligand and receptor proteins, detection and quantification of mRNA expression, amplification of signals, and determination of 3-D structure. Details of protocols with illustrations of results and commentaries are provided throughout the book.
A “blistering exposé” of the USA’s secret history of financial, political, and cultural exploitation of Latin America in the 20th century, with a new introduction (Publishers Weekly). What happened when a wealthy industrialist and a visionary evangelist unleashed forces that joined to subjugate an entire continent? Historians Gerard Colby and Charlotte Dennett tell the story of the forty-year campaign led by Standard Oil scion Nelson Rockefeller and Wycliffe Bible Translators founder William Cameron Townsend to establish a US imperial beachhead in Central and South America. Beginning in the 1940s, future Vice President Rockefeller worked with the CIA and allies in the banking industry to prop up repressive governments, devastate the Amazon rain forest, and destabilize local economies—all in the name of anti-Communism. Meanwhile, Townsend and his army of missionaries sought to undermine the belief systems of the region’s indigenous peoples and convert them to Christianity. Their combined efforts would have tragic and long-lasting repercussions, argue the authors of this “well-documented” (Los Angeles Times) book—the product of eighteen years of research—which legendary progressive historian Howard Zinn called “an extraordinary piece of investigative history. Its message is powerful, its data overwhelming and impressive.”
Hybridization Techniques for Electron Microscopy examines the use of in situ hybridization techniques, including an overview of current perspectives and future developments. The book features in situ methods for fluorescence probes and confocal scanning microscopes. Three in situ hybridization methods for electron microscopes are analyzed: the non-embedded tissue method using ultrathin frozen sections, pre-embedded method, and post-embedded method using material embedded in hydrophilic resin. Positive and negative features are discussed, and clear instructions regarding implementation of techniques are provided. Particular aspects of the techniques are examined in detail, such as preparation of tissue, pretreatment, hybridization procedures, revelation (autoradiography and immunocytology) and checking procedures, in addition to the illustration, interpretation, and discussion of methods and results. The main applications described include virus detection, chromosomal gene mapping, detection of ribosomic nucleic acid, and detection of messenger RNA in animals and plants. Hybridization Techniques for Electron Microscopy is an excellent reference for cytologists, cell biologists, histochemists, cytochemists, molecular endocrinologists, and neuroendocrinologists.
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