Cynthia S. Y. Lee's Books

Next Generation Sequencing in Forensic Science

A Primer

By: Kelly M. Elkins,Cynthia B. Zeller

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Next Generation Sequencing in Forensic Science

A Primer

By: Kelly M. Elkins,Cynthia B. Zeller

Next Generation Sequencing in Forensic Science: A Primer addresses next generation sequencing (NGS) specific to its application to forensic science. The first part of the book offers a history of human identity approaches, including VNTR, RFLP, STR, and SNP DNA typing. It discusses the history of sequencing for human DNA typing, including Sanger sequencing, SNaPshot, pyrosequencing, and principles of next generation sequencing. The chapters present an overview of the forensically focused AmpliSeq, ForenSeq, Precision ID, PowerSeq, and QIAseq panels for human DNA typing using autosomal, Y and X chromosome STRs and SNPs using the MiSeq FGx and Ion Torrent System. The authors outline the steps included in DNA extraction and DNA quantitation that are performed prior to preparing libraries with the NGS kits. The second half of the book details the implementation of ForenSeq and Precision ID to amplify and tag targets to create the library, enrich targets to attach indexes and adaptors, perform library purification and normalization, pool the libraries, and load samples to the cartridge to perform the sequencing on the instrument. Coverage addresses the operation of the MiSeq FGx and Ion Chef, including creating a sample list, executing wash steps, performing NGS, understanding the run feedback files from the instrument, and troubleshooting. ForenSeq and Precision ID panel data analysis are explained, including how to analyze and interpret NGS data and output graphs and charts. The book concludes with mitochondrial DNA (mtDNA) sequencing and SNPs analysis, including the issue of heteroplasmy. The final chapters review forensic applications of microbial DNA, NGS in body fluid analysis, and challenges and considerations for future applications. FEATURES Focuses on human identification using traditional and NGS DNA typing methods targeting short tandem repeats (STRs) Applies the technology and its application to law enforcement investigations and identity and ancestry single nucleotide polymorphisms (SNPs) for investigational leads, mass disaster, and ancestry cases Presents the underlying principles of NGS in a clear, easy-to-understand format for practitioners and students studying DNA in forensic programs This is the first book to prepare practitioners to utilize and implement this new technology in their lab for casework, highlighting early applications of how NGS results have been used in court. The book can be utilized for upper-level undergraduate and graduate students taking courses focused on NGS concepts. Readers are expected to have a basic understanding of molecular and cellular biology and DNA typing.

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Page Count

176

Publisher

CRC Press

Published Date

ISBN 10

1000449254

ISBN 13

9781000449259

Outstanding: Growing Up Asian

By: Cynthia Meng

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Outstanding: Growing Up Asian

By: Cynthia Meng

Witty and humorous, even sarcastic, Cynthia Meng describes and meditates on her high school life as an Asian American. Junior year is about to start. With it come the challenges of SAT and future college applications. Cynthia, a fifteen-year-old American born Chinese, has been asked by her anxious mother to use her "good English" to write a fiction and get published so as to make herself "outstanding" to potential Ivy League admission officers. Highly skeptical and rather unwilling, Cynthia eventually yields to her mother's pressure and does indeed write a book of her own: instead of a fiction, a summer journal that delves into the real life of a typical Asian teenage high-achiever, and those surrounding her. With her fresh perspective, keen observations, smooth and witty writing skill, Cynthia touches upon some major aspects of her middle-class Asian-American family: the absurd frenzy about test scores and which college to go to, the nerve-wracking piano competitions, the pressures of a Chinese community church youth group, the psychological burden from knowing about the sacrifices made by immigrant parents, the contrast between the suburban middle-class and inner city Chinatown, and most importantly, the pervasive desire to define herself in a world of 'fobs' and white-washed Asians. Though a non-fiction narrative, it is quite captivating, sometimes delightful and comical, sometimes lovely and moving. It contains about 60,000 words and a few eye-opening documentary pictures. Cynthia Meng, an American Born Chinese, born in Evanston, IL on October 5, 1993, graduated from Campolindo High School of Moraga, CA in 2011. Cynthia Meng took her SAT in May, 2010 and had a perfect SAT score: 2400. She was the Choir President of Campolindo High School for 2010-2011. Cynthia Meng won Second Place, Senior Division, at Etude Club Young Artist Scholarship Competition 2010, for her piano performance. Cynthia Meng had received admission in 2011 to Harvard, Princeton, Stanford, UPenn, Dartmouth, Northwestern, UC Berkeley and UCLA. She was a winner of National Merit Scholarship Award. Cynthia graduated from Harvard University in 2015.

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Page Count

402

Publisher

Independently Published

Published Date

ISBN 10

109149309X

ISBN 13

9781091493094

The Love of Lotus

By: Cynthia Waiying Wu Wilcox

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The Love of Lotus

By: Cynthia Waiying Wu Wilcox

A young woman's search for faith, purpose, identity, independence and finally happiness"--Page 4 of cover.

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Page Count

233

Publisher

Tyde House Books

Published Date

ISBN 10

0978613589

ISBN 13

9780978613587

Book's by Cynthia S. Y. Lee

Next Generation Sequencing in Forensic Science

Next Generation Sequencing in Forensic Science

Outstanding: Growing Up Asian

Outstanding: Growing Up Asian

The Love of Lotus

The Love of Lotus

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