In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
Until recently, there has been a gap in the scientific literature regarding women's health issues. The National Institutes of Health are encouraging more research focused on women, and many researchers are beginning to answer the call. Nutrition and Women's Cancers presents a comprehensive discussion of the etiological factors relevant to cancers that are most common in women. It covers female-specific cancers as well as lung and colon cancer, which are highly prevalent in women. The text focuses on the influence of nutrition and diet on these cancers and also discusses the impact of genetics and environmental factors, so that the reader may interpret the relative importance of diet in the complete context of multiple causes. This book presents specific studies of women's health and cancer issues and offers directions for future research in the field.
During an era when many women concentrated on hearth and home, thousands of women quietly and without pay served in law enforcement. They organized, administered, presented reports to county commissioners, prepared for inspections, comforted victims, disciplined unruly inmates, fought with escapees, rode shotgun with their husbands as backup, and raised children, tended gardens, and kept house. They risked their lives every day and some paid the ultimate price. This is their story. The office of county sheriff has existed in America since 1634. Between 1800 and 1960, families of the sheriff lived in or near the jail. All family members, young and old, worked alongside the lawman to fulfill the required duties, without additional pay. The mom and pop jail was truly a family business. After the middle of the 20th century, fewer families carried on this tradition as counties modernized and jails became professionalized.
Here is the most comprehensive and up-to-date treatment of one of the hottest areas of chemical research. The treatment of fundamental kinetics and photochemistry will be highly useful to chemistry students and their instructors at the graduate level, as well as postdoctoral fellows entering this new, exciting, and well-funded field with a Ph.D. in a related discipline (e.g., analytical, organic, or physical chemistry, chemical physics, etc.). Chemistry of the Upper and Lower Atmosphere provides postgraduate researchers and teachers with a uniquely detailed, comprehensive, and authoritative resource. The text bridges the "gap" between the fundamental chemistry of the earth's atmosphere and "real world" examples of its application to the development of sound scientific risk assessments and associated risk management control strategies for both tropospheric and stratospheric pollutants. - Serves as a graduate textbook and "must have" reference for all atmospheric scientists - Provides more than 5000 references to the literature through the end of 1998 - Presents tables of new actinic flux data for the troposphere and stratospher (0-40km) - Summarizes kinetic and photochemical date for the troposphere and stratosphere - Features problems at the end of most chapters to enhance the book's use in teaching - Includes applications of the OZIPR box model with comprehensive chemistry for student use
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
An omnibus collection of three favorites by the best-selling author of 'A Woman of Substance' includes complete copies of 'Hold the Dream', 'To Be the Best', and 'Act of Will' in one romantic volume.
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