Azza El Amir's Books

Effects of Protein Purification Techniques on the Immune Response

By: Azza El Amir

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Effects of Protein Purification Techniques on the Immune Response

By: Azza El Amir

This study discusses a very important issue in immune responses against different human diseases. Different immunological methods are to be used to evaluate the immunogenicity of diphtheria toxin (DT) purified by various techniques, thus it would be possible to delineate the best purification technique that yields a better immunogen to be used as a vaccine. Evaluation of the immunogenicity of the vaccine is by assessment of its ability to induce profound humoral and cellular immune responses.

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Page Count

101

Publisher

diplom.de

Published Date

ISBN 10

3954894343

ISBN 13

9783954894345

Immunological and Environmental Studies On Giardia Duodenalis

Giardiasis

By: Alyaa Farid,Azza El Amir

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Immunological and Environmental Studies On Giardia Duodenalis

Giardiasis

By: Alyaa Farid,Azza El Amir

Giardia duodenalis (synonyms G. lamblia or G. intestinalis), the only species belonging to the genus Giardia found in humans, is a pathogenic protozoan with a worldwide distribution having a more relevant prevalence in warm climate and in children. G. duodinalis is a water-borne flagellated parasite that causes giardiasis in humans. In addition, giardiasis affects domestic and wild mammals “e.g., cats, dogs, cattle, deer, and beavers” (Thompson, 2000). Host-to-host transference of Giardia cysts takes place via the fecal-oral route. This allows the protozoan to infect the next generation of hosts through a diverse range of mechanisms: person to person, animal to human or by contact with contaminated water and food. Outbreaks commonly occur in areas with inadequate water treatment, especially developing countries, where infection rates can be higher than 50% of the total population. It is well documented that in developing countries, infections are associated with poor sanitary conditions, poor water quality and overcrowding. Giardia is one of the leading protozoan causes of gastrointestinal illness worldwide and has joined the ranks of the WHO Neglected Diseases Initiative. Giardiasis constitutes an important public health problem in Egyptian children. Contamination of the Nile River with fecal materials including viruses and pathogenic protozoa still represents an environmental health hazard in Egypt, especially in rural areas. The clinical features of giardiasis range from acute or chronic diarrhea, abdominal pain, nausea, flatulence, vomiting, weight loss, to absence of symptoms and signs. In the diagnosis of Giardia, stool examination is the traditional, safest and easiest method. Microscopy of Giardia cysts and trophozoites is more straightforward, and there is little risk of confusion with other parasites. Moreover, only “ghost” cysts with an empty appearance are sometimes not recognized as Giardia parasites. However, the sensitivity of microscopy is quite low due to the intermittent excretion of Giardia cysts, the microscopical examination of a single stool specimen has a low sensitivity and may therefore miss up to 50% of Giardia infections. And thus, it is recommended that at least three samples be examined in order to rule out giardiasis which is time consuming and delay the diagnosis.

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Anchor Academic Publishing

Published Date

ISBN 10

395489467X

ISBN 13

9783954894673

Parasitic Antigens for Vaccine Development

By: Azza El Amir

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Parasitic Antigens for Vaccine Development

By: Azza El Amir

Despite effective chemotherapy, fascioliasis remains a major public health problem in developing countries, with at least 17 million active infections, resulting in significant morbidity, late infection detection, and rapid reinfection after treatment. Therefore, alternative control strategies are mandatory. Proteases, myofibrillar proteins, are found only in invertebrates. In the present study, adult fresh Fasciola gigantica (F. gigantica) worms were homogenized; an antigen was purified and used to raise rabbit polyclonal antibodies (pAb). The purified pAb was then used in sandwich ELISA to detect Fasciola antigens in sera samples from a total 135 cattle.

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Anchor Academic Publishing

Published Date

ISBN 10

3960675542

ISBN 13

9783960675549

Early Hydatid Diagnosis: A Problem to be solved

Hydatidosis

By: Alyaa Farid,Azza El Amir

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Early Hydatid Diagnosis: A Problem to be solved

Hydatidosis

By: Alyaa Farid,Azza El Amir

Human cystic hydatid disease (CHD) caused by the larval stage (hydatid cyst) of the dog tapeworm, E. granulosus, is a major infection with worldwide distribution and variable geographical incidence. The present study evaluates the two most popular techniques, sandwich ELISA and Latex techniques for the diagnosis of human hydatidosis by antigen detection. In the present study, crude protoscolex antigen (CPA) detection was carried out through several steps including the preparation of protoscoleces antigen, production; purification and labeling of rabbit IgG pAb. The Latex technique and ELISA tests have been found to be high sensitive and suitable for the antigen detection of cystic hydatid disease. In conclusion, antigen detection assay is superior and more sensitive than antibody detection assay, especially in diagnosing active infection where hydatid cysts are predominant. Antigen detection assay might be a useful approach for assessment of the efficacy of treatment especially after removal of the cyst. Further studies are recommended to improve the diagnostic efficacy of antigen based ELISA method. Sandwich ELISA and Latex techniques appear to be sufficiently sensitive assays for the detection of human echinococcosis.

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Page Count

129

Publisher

Anchor Academic Publishing

Published Date

ISBN 10

3954894688

ISBN 13

9783954894680

Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

By: Azza El Amir

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Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

By: Azza El Amir

Doctoral Thesis / Dissertation from the year 2014 in the subject Medicine - Biomedical Engineering, Cairo University (Faculty of Science), course: Immunology, language: English, abstract: P. aeruginosa is a gram-negative bacterium that causes a variety of diseases in compromised hosts. Bacterial endotoxins such as LPS are the major outer surface membrane components present in almost all gram-negative bacteria and act as extremely strong stimulators of innate immunity and inflammation of the airway. The present study was undertaken to determine the effect of combined administration of Gentamicin (GENT) as antibiotic and Dexamethasone (DEXA) as ant-inflammatory drug on some physiological, immunological and histological parameters. After determination of LD50 of P. aeruginosa, mice groups were injected with DEXA, GENT and LPS alone or in combination. LPS single injection caused a significant increase of total protein, globulin, total leukocyte count, lymphocytes, neutrophils and level of IgM and IgG. DEXA induced an increase of serum total lipid, ALT and AST levels, neutrophilia and lymphopenia. GENT administration increased serum total protein, globulin, total lipid, ALT and AST levels. Physiological and immunological examination demonstrated that combined treatment has a significant effect as decreaseing serum total protein, globulin, lymphocytes and IgG level than single treatment. Histological examination demonstrated that the inflammation of thymus, spleen, lymph node and liver decreases in mice received combined treatment than those received individual treatment. Concurrent administration of DEXA and GENT has greatest effect in protecting organs against damage in case of endotoximia.

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Page Count

120

Publisher

GRIN Verlag

Published Date

ISBN 10

3656697612

ISBN 13

9783656697619

Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

By: Azza El Amir

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Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

By: Azza El Amir

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120

Publisher

GRIN Verlag

Published Date

ISBN 10

3656697612

ISBN 13

9783656697619

Parasitic Antigens for Vaccine Development

By: Azza El Amir

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Parasitic Antigens for Vaccine Development

By: Azza El Amir

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Anchor Academic Publishing

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ISBN 10

3960675542

ISBN 13

9783960675549

Book's by Azza El Amir

Effects of Protein Purification Techniques on the Immune Response

Effects of Protein Purification Techniques on the Immune Response

Immunological and Environmental Studies On Giardia Duodenalis

Immunological and Environmental Studies On Giardia Duodenalis

Parasitic Antigens for Vaccine Development

Parasitic Antigens for Vaccine Development

Early Hydatid Diagnosis: A Problem to be solved

Early Hydatid Diagnosis: A Problem to be solved

Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

Effect of concurrent administration of an antibiotic and an anti-inflammatory drug on the immunotoxicity of bacterial endotoxins

Parasitic Antigens for Vaccine Development

Parasitic Antigens for Vaccine Development

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